Cytochrome P450 119 Compounds I Formed by Chemical Oxidation and Photooxidation Are the Same Species.
Cytochrome P450 119 Compounds I Formed by Chemical Oxidation and Photooxidation Are the Same Species.
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化学氧化和光氧化形成的细胞色素 P450 119 化合物 I 是同一物种。
DOI：
10.1002/chem.201202254
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Newcomb,Martin
中科院分区：
文献类型：
--
作者：
Su,Zhi;Horner,JohnH;Newcomb,Martin
以细胞色素P450 119为原料，经静息酶经过氧亚硝酸盐氧化生成化合物II，再经光氧化生成化合物I，并与静息酶经m -氯过氧苯甲酸（MCPBA）氧化生成的化合物I进行了比较。两种方法的紫外/可见光谱相同，氧化月桂酸和棕榈酸及其（ω‐2,ω‐2,ω‐3,ω‐3）‐四氘类似物的产物相同，氧化月桂酸和辛酸的动力学相同。两种方法所产生的瞬态的实验特性表明，两种方法所形成的化合物I是相同的。
Compound I from cytochrome P450 119 prepared by the photooxidation method involving peroxynitrite oxidation of the resting enzyme to Compound II followed by photooxidation to Compound I was compared to Compound I generated bym‐chloroperoxybenzoic acid (MCPBA) oxidation of the resting enzyme. The two methods gave the same UV/Visible spectra, the same products from oxidations of lauric acid and palmitic acid and their (ω‐2,ω‐2,ω‐3,ω‐3)‐tetradeuterated analogues, and the same kinetics for oxidations of lauric acid and caprylic acid. The experimental identities between the transients produced by the two methods leave no doubt that the same Compound I species is formed by the two methods.