Autologous adipose-derived regenerative cells are effective for chronic intractable radiation injuries
Autologous adipose-derived regenerative cells are effective for chronic intractable radiation injuries
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DOI：
10.1093/rpd/ncs176
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发表时间：
2012-10-01
影响因子：
1
通讯作者：
Yamashita, S.
中科院分区：
文献类型：
--
作者：
Akita, S.;Yoshimoto, H.;Yamashita, S.
对慢性放射性损伤（如溃疡）的有效治疗容易发生感染。僵硬是预期的，因为治疗辐射通常涉及更宽和更深的组织，并且通常需要广泛的清创和重建，这有时不适合老年和受损的宿主。自体脂肪源性再生细胞（ADRCs）产量高，形成相对老年的连续10例病例，63.6 ± 14.9岁（5289岁），平均辐射剂量为75.0 ± 35.4戈伊（50 ± 120戈伊），至少随访10个月。通过临床以及通过计算机断层扫描和磁共振成像的成像，测试伤口床和边缘中的最小清创和ADRC注射沿着外周中的脂肪和ADRC的混合物的注射的功效和再生组织质量。获得1.61.310（7）细胞的未培养ADRC。术后6 ~ 3.2周（210周）全部愈合。完成部位的发病率可以忽略不计，没有重大并发症，如瘫痪或大面积血肿。再生组织质量明显上级术前，与完整组织连接的脂肪和ADRC混合物非常柔软和柔韧。平均随访时间为1.90.8年（0.92.9年），显示治疗后无复发或新发溃疡。因此，ADRCs治疗长达数十年的放射损伤是有效的，安全的，并改善了伤口的质量。
Effective therapy for chronic radiation injuries, such as ulcers, is prone to infection. Stiffness is expected since the therapeutic radiation often involves wider and deeper tissues and often requires extensive debridement and reconstruction, which are not sometimes appropriate for elderly and compromised hosts. Autologous adipose-derived regenerative cells (ADRCs) are highly yielding, forming relatively elderly aged consecutive 10 cases, 63.614.9 y (5289 y), with mean radiation dose of 75.035.4 Gy (50120 Gy) were included with at least 10-month follow-up. Minimal debridement and ADRC injection in the wound bed and margin along with the injection of mixture of fat and ADRCs in the periphery were tested for efficacy and regenerated tissue quality by clinically as well as imaging by computed tomography and magnetic resonance imaging. Uncultured ADRCs of 1.61.310(7) cells were obtained. All cases healed uneventfully after 6.63.2 weeks (210 weeks) post-operatively. The done site morbidity was negligible and without major complications, such as paralysis or massive haematoma. The regenerated tissue quality was significantly superior to the pre-operative one and the mixture of fat and ADRCs connected to the intact tissue was very soft and pliable. Mean follow-up at 1.90.8 y (0.92.9 y) revealed no recurrence or new ulceration after treatment. Thus, the ADRCs treatment for decades-long radiation injuries is effective, safe and improves the quality of wounds.