Hypersensitivity to tobacco antigen.
Hypersensitivity to tobacco antigen.
复制标题
对烟草抗原过敏。
DOI：
10.1073/pnas.73.5.1712
复制
发表时间：
1976
影响因子：
11.1
通讯作者：
H. Wiedemann
中科院分区：
文献类型：
--
作者：
C. G. Becker;T. Dubin;H. Wiedemann
用硫酸铵分级沉淀、SephadexG-25柱层析和聚丙烯酰胺凝胶连续流动制备电泳，从烤烟盐水提取液中纯化出一种分子量为18，000的糖蛋白。31名志愿者中有12名（6/15名吸烟者和6/16名非吸烟者）在皮内注射2 μ g该材料时表现出速发型皮肤超敏反应（风团和潮红反应）。在香烟烟雾冷凝物和香烟烟雾中证明并纯化了免疫化学相似的物质。香烟烟雾冷凝物（“焦油”）中的浓度测定为1.8-3.6 mg/g。在茄子、绿色辣椒、马铃薯和番茄中也发现了抗原交叉反应性物质，这些植物和烟草一样都是茄科植物。
A glycoprotein of molecular weight 18,000 was purified from saline extracts of cured tobacco leaves by ammonium sulfate fractionation, chromatography on Sephadex G-25 and continuous-flow preparative electrophoresis on polyacrylamide gel. Twelve of 31 volunteers (6/15 smokers and 6/16 nonsmokers) exhibited immediate cutaneous hypersensitivity (wheal and flare reactions) when injected intracutaneously with 2 mug of this material. Immunochemically similar material was demonstrated in, and purified from, cigarette smoke condensate and cigarette smoke. The concentration in cigarette smoke condensate ("tar") was determined to be 1.8-3.6 mg/g. Antigenically crossreactive material was also demonstrated in eggplants, green peppers, potatoes, and tomatoes, which, like tobacco, are members of the family Solanaceae.