Controlling the Cyanobacterial Clock by Synthetically Rewiring Metabolism.
Controlling the Cyanobacterial Clock by Synthetically Rewiring Metabolism.
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DOI：
10.1016/j.celrep.2015.11.031
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发表时间：
2015-12-22
期刊：
影响因子：
8.8
通讯作者：
Rust MJ
中科院分区：
文献类型：
--
作者：
Pattanayak GK;Lambert G;Bernat K;Rust MJ
生物钟是一种振荡系统，使生物体能够预测环境中的节律变化。几项研究表明，生物钟与新陈代谢有关，但通常还不清楚代谢信号是影响生物钟的众多声音中的一个，还是代谢周期是主要的时钟同步器。为了解决蓝藻中的这个问题，我们使用了一种合成生物学的方法来使正常的自养细胞能够在外源糖上生长。这使我们能够独立于光明和黑暗操纵新陈代谢。我们发现，给培养物加糖会阻止暗脉冲的时钟重置效应。此外，在没有光线的情况下，时钟有效地与有节奏的摄食驱动的新陈代谢周期同步。我们得出结论，代谢活性与其来源无关，是蓝藻的主要时钟驱动因素。
Circadian clocks are oscillatory systems and allow organisms to anticipate rhythmic changes in the environment. Several studies have shown that circadian clocks are connected to metabolism, but it is not generally clear whether metabolic signaling is one voice among many that influence the clock, or whether metabolic cycling is the major clock synchronizer. To address this question in cyanobacteria, we used a synthetic biology approach to make normally autotrophic cells capable of growth on exogenous sugar. This allowed us to manipulate metabolism independently from the light and dark. We found that feeding sugar to cultures blocked the clock-resetting effect of a dark pulse. Further, in the absence of light, the clock efficiently synchronizes to metabolic cycles driven by rhythmic feeding. We conclude that metabolic activity, independent of its source, is the primary clock driver in cyanobacteria.