In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb
In vivo odourant response properties of migrating adult-born neurons in the mouse olfactory bulb
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DOI：
10.1038/ncomms7349
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发表时间：
2015-02-01
影响因子：
16.6
通讯作者：
Garaschuk, Olga
中科院分区：
文献类型：
--
作者：
Kovalchuk, Yury;Homma, Ryota;Garaschuk, Olga
哺乳动物嗅球的球旁神经元（JGN）在整个生命过程中产生。它们整合到预先存在的神经网络中，它们在其中的分化和存活取决于感觉活动，但是这些成年细胞何时以及如何获得对感觉刺激的反应性仍然是未知的。逆转录病毒标记的成人出生的JGN的体内双光子成像显示，在注射后（DPI）7天后，接近90%的细胞到达肾小球层。到达后，成年出生的JGN仍在迁移，但在DPI 9时，其中52%具有气味诱发的Ca2+信号。它们对气味的敏感性与母体肾小球和周围的JGN非常相似，它们的自发和气味诱发的尖峰与它们的居民邻居相似。我们的数据显示，成人出生的细胞在功能上非常快速地整合到预先存在的神经网络中。这些细胞的成熟模式的气味诱发的反应强烈的对比，其分子表型，这是典型的不成熟的，迁移的神经母细胞。
Juxtaglomerular neurons (JGNs) of the mammalian olfactory bulb are generated throughout life. Their integration into the preexisting neural network, their differentiation and survival therein depend on sensory activity, but when and how these adult-born cells acquire responsiveness to sensory stimuli remains unknown. In vivo two-photon imaging of retrovirally labelled adult-born JGNs reveals that similar to 90% of the cells arrive at the glomerular layer after day post injection (DPI) 7. After arrival, adult-born JGNs are still migrating, but at DPI 9, 52% of them have odour-evoked Ca2+ signals. Their odourant sensitivity closely resembles that of the parent glomerulus and surrounding JGNs, and their spontaneous and odour-evoked spiking is similar to that of their resident neighbours. Our data reveal a remarkably rapid functional integration of adult-born cells into the preexisting neural network. The mature pattern of odour-evoked responses of these cells strongly contrasts with their molecular phenotype, which is typical of immature, migrating neuroblasts.