HYPOXIC FRACTION DETERMINATIONS WITH THE BA1112 RAT SARCOMA - VARIATION WITHIN AND AMONG ASSAY TECHNIQUES
HYPOXIC FRACTION DETERMINATIONS WITH THE BA1112 RAT SARCOMA - VARIATION WITHIN AND AMONG ASSAY TECHNIQUES
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DOI：
10.2307/3576486
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发表时间：
1984-01-01
影响因子：
3.4
通讯作者：
MARTIN, DF
中科院分区：
文献类型：
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作者：
MOULDER, JE;MARTIN, DF
在同一肿瘤系统中，用不同的测定技术测定低氧分数往往会得到不相容的结果。通过3种检测技术测量BA 1112肉瘤的缺氧部分：肿瘤控制和生长延迟试验（肿瘤在照射后保持原位）和配对生存曲线试验（肿瘤在照射后切除）。在麻醉和不麻醉的情况下，对生长在2个不同皮下部位的肿瘤进行了检测。在肿瘤对照实验中，对宿主进行麻醉可以显著降低计算出的缺氧分数，但在成对生存实验中则没有。与肿瘤控制或生长延迟试验相比，切除试验始终给出较高的缺氧分数估计。讨论了在切除法中产生比原位法更高的低氧组分的一些因素。由于生长延迟和肿瘤控制试验之间的差异以及重复测定之间的差异，结果的解释变得复杂。测定结果之间的差异几乎完全是由人工缺氧肿瘤反应的变化引起的，而不是由有氧肿瘤反应的变化引起的。通常在测量低氧分数时所做的假设可能无效。
Measurement of the hypoxic fraction in the same tumor system by different assay techniques often gives incompatible results. The hypoxic fraction of the BA 1112 sarcoma was measured by 3 assay techniques: tumor control and growth delay assays in which the tumors remain in situ after irradiation, and paired survival curve assays in which the tumors are excised after irradiation. The assays were done with and without anesthesia on tumors growing in 2 different subcutaneous sites. Anesthesia of the hosts produced a statistically significant decrease in the calculated hypoxic fraction in a tumor control assay, but not in a paired survival assay. The excision assays gave consistently higher hypoxic fraction estimates than either the tumor control or growth delay assays. Some of the factors which could produce higher hypoxic fractions in excision assays than in in situ assays are discussed. Interpretation of the results was complicated by disagreements between the growth delay and tumor control assays, and by disparities between replicate determinations. The discrepancies among assays are caused almost entirely by variations in the response of the artificially hypoxic tumors, rather than variations in the response of aerobic tumors. Assumptions commonly made when measuring hypoxic fractions may not be valid.