Heterogeneity of mouse vascular endothelium. In vitro studies of lymphatic, large blood vessel and microvascular endothelial cells.
Heterogeneity of mouse vascular endothelium. In vitro studies of lymphatic, large blood vessel and microvascular endothelial cells.
复制标题
小鼠血管内皮的异质性。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
R. Auerbach
中科院分区：
文献类型：
--
作者：
F. Gumkowski;G. Kaminska;M. Kaminski;L. Morrissey;R. Auerbach
已从小鼠的肺、肝、脑、心、胎盘、肾、膀胱、乳腺、卵巢和附睾脂肪垫建立了微血管内皮细胞培养物。此外，还从小鼠的主动脉和胸导管获得了大血管内皮细胞。通过流式细胞术测定血管紧张素转换酶、乙酰低密度脂蛋白受体的差异存在、细胞表面抗原的可变表达以及各种植物凝集素的不同结合，显示了这些细胞的异质性。我们开发的内皮细胞系为在长期作为生物医学研究关键物种的小鼠中研究血管内皮的广泛生物学功能和特性提供了手段。
Microvascular endothelial cell cultures have been established from mouse lung, liver, brain, heart, placenta, kidney, urinary bladder, mammary gland, ovary and epididymal fat pad. In addition, large vessel endothelial cells have been obtained from the mouse aorta and thoracic duct. The heterogeneity of these cells has been shown by flow cytometric determination of angiotensin-converting enzyme, by differential presence of the acetyl low density lipoprotein receptor, by the variable expression of cell surface antigens, and by differential binding of various plant lectins. The endothelial cell lines we have developed provide the means to examine in the mouse, long a key species for biomedical research, a wide range of biological functions and properties of the vascular endothelium.