Selective adsorption of bilirubin against albumin to alkylamine functionalized PVA microspheres
Selective adsorption of bilirubin against albumin to alkylamine functionalized PVA microspheres
复制标题
烷基胺功能化 PVA 微球选择性吸附胆红素和白蛋白
DOI：
10.1080/09205063.2018.1553104
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发表时间：
2019-03-24
影响因子：
3.6
通讯作者：
Ou, Lailiang
中科院分区：
文献类型：
--
作者：
Ma, Yingda;Chen, Jian;Ou, Lailiang
摘要 吸附剂广泛应用于血液灌流中去除胆红素。然而，它们的性能常常因血浆蛋白的存在而受到影响。在本研究中，研究了用不同氨基烷烃配体功能化的聚乙烯醇微球（PVAm）的胆红素吸附能力，目的是获得对白蛋白的结合选择性。与临床吸附剂 BPR（PBS 中的 92% 和 4.84 mg/mL，PBS 中的 71% 和 3.80 mg/mL，白蛋白溶液中的 3.80 mg/mL）相比，辛胺功能化 PVA 微球（PVAm-8）表现出优异的胆红素吸附能力（PBS 中为 75% 和 3.95 mg/mL，白蛋白溶液中为 72% 和 3.84 mg/mL）。 白蛋白溶液）。 PVAm-8 的胆红素吸附能力很大程度上不受白蛋白存在的影响。胆红素对PVAm-8的吸附主要通过疏水效应发生，在PBS和白蛋白溶液中的吸附均符合单层模型和伪一级模型。 PVAm-8对溶血活性、血液成分稳定性和凝血活性的影响可以忽略不计，表明PVAm-8作为血液灌流应用的高亲和力胆红素吸附剂具有良好的潜力。图解摘要
Abstract Adsorbents are widely used in hemoperfusion for bilirubin removal. However, their performance is often compromised by the presence of plasma proteins. In this study, the bilirubin adsorption capacity of polyvinyl alcohol microspheres (PVAm) functionalized with different amino-alkane ligands has been investigated, with the aim of gaining binding selectivity over albumin. Octylamine-functionalized PVA microspheres (PVAm-8) exhibited an excellent adsorption capacity for bilirubin (75% and 3.95 mg/mL in PBS vs 72% and 3.84 mg/mL in albumin solution) when compared to the clinical adsorbent BPR (92% and 4.84 mg/mL in PBS vs 71%, and 3.80 mg/mL in albumin solution). The bilirubin adsorption capacities of PVAm-8 were largely unaffected by the presence of albumin. Adsorption of bilirubin to PVAm-8 occurs mainly through hydrophobic effects, with adsorption consistent with the monolayer model and the pseudo-first-order model operating in both PBS and albumin solution. The effects of PVAm-8 on hemolytic activity, blood component stability and coagulant activity were negligible, indicating that PVAm-8 has good potential as a high-affinity bilirubin adsorbent for hemoperfusion applications. Graphical Abstract