IGE AND EOSINOPHIL REGULATION IN A MURINE MODEL OF ALLERGIC ASPERGILLOSIS
IGE AND EOSINOPHIL REGULATION IN A MURINE MODEL OF ALLERGIC ASPERGILLOSIS
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DOI：
10.1002/jlb.56.5.593
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发表时间：
1994-11-01
影响因子：
5.5
通讯作者：
COFFMAN, RL
中科院分区：
文献类型：
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作者：
KURUP, VP;CHOI, HY;COFFMAN, RL
BALB/c小鼠暴露于烟曲霉菌（Af）（导致人类过敏性支气管肺曲霉病的抗原），导致血清免疫球蛋白E（IgE）水平升高，外周血和肺嗜酸性粒细胞增多症与人类疾病中观察到的相似。我们已经研究了白细胞介素-4（IL-4）、IL-5和干扰素-γ在调节小鼠模型中IgE和嗜酸性粒细胞增多中的作用。通过腹膜内注射吸附到明矾上的可溶性Af抗原免疫动物。这些动物出现IgE和Af特异性IgG 1升高。然后在通过鼻内途径最终暴露于颗粒Af抗原之前用抗细胞因子单克隆抗体处理。结果显示，抗IL-5消除了小鼠中的嗜酸性粒细胞增多症，而用抗IL-4治疗的小鼠与治疗前水平相比保持相同或降低的IgE水平。所有抗-IL-5、抗-IFN-γ和对照抗体处理的动物显示出增强的IgE水平。与所有其他组相比，小鼠的抗IFN-γ治疗导致嗜酸性粒细胞增多症显著增强。在肺组织学切片中观察到的嗜酸性粒细胞数量证实了在外周血中检测到的嗜酸性粒细胞增多。这些结果表明，在BALB/c小鼠暴露于Af抗原后，IgE和嗜酸性粒细胞的增加是由于Af诱导的IL-4和IL-5的产生，IgE和嗜酸性粒细胞的独立调节。
Exposure of BALB/c mice to Aspergillus fumigatus (Af), the antigen responsible for causing allergic bronchopulmonary aspergillosis in humans, caused elevated levels of serum immunoglobulin E (IgE) and peripheral blood and lung eosinophilia similar to that observed in the human disease. We have investigated the role of interleukin-4 (IL-4), IL-5 and interferon-gamma in regulating IgE and eosinophilia in the mouse model. Animals were immunized by intraperitoneal injections of soluble Af antigens adsorbed to alum. These animals developed elevated IgE and Af specific IgG1. and were then treated with anticytokine monoclonal antibodies before the final exposure to particulate Af antigens by the intranasal route. The results showed that anti-IL-5 abrogated eosinophilia in mice, while those treated with anti-IL-4 retained the same or reduced IgE levels compared to pretreatment levels. All anti-IL-5, anti-IFN-gamma, and control antibody-treated animals showed enhanced IgE levels. Anti-IFN-gamma treatment of mice resulted in marked enhancement of eosinophilia compared to all other groups. Eosinophil numbers observed in the histological sections of the lungs confirmed the eosinophilia detected in the peripheral blood. These results indicate that the increase in IgE and eosinophils after exposure to Af antigens in BALB/c mice are due to Af-induced production of IL-4 and IL-5 and that both IgE and eosinophilia are independently regulated.