Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi
Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi
复制标题
使用 rDNA T-RFLP 进行木材腐烂真菌的检测和物种区分
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
C. Land
中科院分区：
文献类型：
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作者：
Ulrika Råberg;N. Högberg;C. Land
摘要本研究利用PCR技术对木材腐朽的早期阶段进行了检测，并与显微镜检测进行了比较。木材腐朽菌Postia胎盘和Coniophora puteana检测内部木材样品的末端限制性片段长度多态性（T-RFLP）培养2周后，与单一培养，而菌丝的显微镜检测是不可能的，直到7周后。利用rDNA的T-RFLPs研究真菌群落时的一个潜在问题是种内变异使数据分析复杂化。在这项工作中，我们表明，在Coniophora puteana的rDNA的内部转录间隔区的种内序列变异允许T-RFLP鉴定这个物种。这是由于片段长度的种内变异，以及所选限制性位点中不存在点突变。
Abstract In the present work PCR technology was used as a tool to detect the early stages of wood decay and was compared with microscopic evaluation. The wood decay fungi Postia placenta and Coniophora puteana were detectable in interior wood samples by terminal restriction fragment length polymorphism (T-RFLP) after 2weeks of incubation with monocultures, while microscopic detection of hyphae was not possible until after 7 weeks. A potential problem when fungal communities are studied with T-RFLPs of rDNA is that intra-specific variation complicates data analysis. In this work, we show that intra-specific sequence variation in the internal transcribed spacer of the rDNA in Coniophora puteana allows T-RFLP identification of this species. This is due to intra-specific variations in fragment length, in combination with the absence of point mutations in the selected restriction sites.