A GAL4 Driver Resource for Developmental and Behavioral Studies on the Larval CNS of Drosophila
A GAL4 Driver Resource for Developmental and Behavioral Studies on the Larval CNS of Drosophila
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DOI：
10.1016/j.celrep.2014.06.065
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发表时间：
2014-08-07
期刊：
影响因子：
8.8
通讯作者：
Truman, James W.
中科院分区：
文献类型：
--
作者：
Li, Hsing-Hsi;Kroll, Jason R.;Truman, James W.
我们报告的幼虫中枢神经系统的表达模式为6,650 GAL 4线的基础上顺式调控区（CRM）从果蝇基因组。成人中枢神经系统的表达模式，以前报道了这个集合，从而提供了一个独特的资源，确定成人细胞的起源。一个说明性的例子揭示了腹侧中枢神经系统的星形胶质细胞样胶质细胞的起源。除了幼虫的神经元和神经胶质细胞，幼虫中枢神经系统包含分散的谱系的未成熟的，成人特定的神经元。在这个大的CRM集合内的谱系表达的比较提供了用于指定神经元类型的代码的洞察。CRM编码谱系表达的密集和稀疏模式。在稀疏表达模式中，大脑和胸部谱系之间几乎没有相关性，但在密集模式中，这两个区域的表达高度相关。相比之下，视叶在发育过程中似乎使用了一套不同的遗传指令。
We report the larval CNS expression patterns for 6,650 GAL4 lines based on cis-regulatory regions (CRMs) from the Drosophila genome. Adult CNS expression patterns were previously reported for this collection, thereby providing a unique resource for determining the origins of adult cells. An illustrative example reveals the origin of the astrocyte-like glia of the ventral CNS. Besides larval neurons and glia, the larval CNS contains scattered lineages of immature, adult-specific neurons. Comparison of lineage expression within this large collection of CRMs provides insight into the codes used for designating neuronal types. The CRMs encode both dense and sparse patterns of lineage expression. There is little correlation between brain and thoracic lineages in patterns of sparse expression, but expression in the two regions is highly correlated in the dense mode. The optic lobes, by comparison, appear to use a different set of genetic instructions in their development.