Microtubules and vesicles under controlled tension
Microtubules and vesicles under controlled tension
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DOI：
10.1103/physreve.55.850
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发表时间：
1997-01-01
影响因子：
2.4
通讯作者：
Libchaber, A
中科院分区：
文献类型：
--
作者：
Fygenson, DK;Elbaum, M;Libchaber, A
微管被困在细胞大小的囊泡（直径约为10 μ m）中，其形状让人联想到活细胞。在这项工作中，研究了三种结构：单个微管、线性束和环形带。机械性能的探讨，通过改变囊泡膜张力，使用微量吸管抽吸。定量结果包括测量单个微管的持久长度，6.3+/-2.4 mm，以及囊泡膜的拉伸模量，k（s）大于或等于140 erg/cm（2）。微管束的数目约为100个，微管带的总长度约为0.5cm。观察到了张力诱导的一级相变。弯曲丛的稳定性的损失与鞍结分岔。
Microtubules trapped inside cell-sized vesicles (similar to 10-mu m diameter) define shapes reminiscent of living cells. In this work, three configurations are studied: an individual microtubule, a linear bundle, and a circular band. Mechanical properties are probed by varying the vesicle membrane tension using micropipet aspiration. Quantitative results include measurements of the persistence length of a single microtubule, 6.3+/-2.4 mm, and the stretching modulus of the vesicle membrane, k(s) greater than or equal to 140 erg/cm(2). The number of microtubules in a typical bundle is similar to 100, and the total length in a typical band is similar to 0.5 cm, A tension-induced first-order transition from linear bundle to circular band is observed and modeled. The loss of stability of the bent bundle is associated with a saddle-node bifurcation.