Anti-Neoplastic Cytotoxicity of SN-38-Loaded PCL/Gelatin Electrospun Composite Nanofiber Scaffolds against Human Glioblastoma Cells In Vitro
Anti-Neoplastic Cytotoxicity of SN-38-Loaded PCL/Gelatin Electrospun Composite Nanofiber Scaffolds against Human Glioblastoma Cells In Vitro
复制标题
负载 SN-38 的 PCL/明胶电纺复合纳米纤维支架对人胶质母细胞瘤细胞的体外抗肿瘤细胞毒性
DOI：
10.1002/jps.24684
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发表时间：
2015
影响因子：
3.8
通讯作者：
ong
中科院分区：
文献类型：
--
作者：
Zhu Xiaodong;Ni Shilei;Xia Tongliang;Yao Qingyu;Li Haoyuan;Wang Benlin;Wang Jiangang;Li Xingang;Su W;ong
制备了聚己内酯（PCL）/明胶（GT）电纺支架，用于7-乙基-10-羟基喜树碱（SN-38）的缓释。引入乙酸以改善PCL和GT的相容性以产生均匀的相容膜混合物。研究了SN-38在二元混合物中的含量对可加工性、纤维形态、吸水性、溶胀性和药物释放的影响。静电纺丝PCL/GT共混物非织造纤维显示纤维表面粗糙度，降低PCL结晶度，并增加溶胀与增加药物含量的1，2，和4重量%。此外，增加SN-38的浓度降低了GT的降解速率。此外，我们假设在单轴纤维中存在药物含量饱和点，以解释PG 2与PG 1和PG 4相比的不同药物释放模式。该基质具有良好的生物降解性和抗肿瘤作用。我们的研究结果表明，SN-38负载PCL/GT纤维可以通过静电纺丝获得。装载SN-38的纤维值得进一步评价，作为潜在预防手术肿瘤切除后局部复发的手段。
Electrospun poly(ε-caprolactone) (PCL)/gelatin (GT) scaffolds were developed to provide controlled release of 7-ethyl-10-hydroxy camptothecin (SN-38). Acetic acid was introduced to improve the miscibility of PCL and GT to produce a homogeneous nanofiber membrane mixture. The effect of SN-38 content in binary mixtures on processability, fiber morphology, water sorption, swelling, and drug release was investigated. Electrospun PCL/GT blend nonwoven fibers showed fiber surface roughness, decreased PCL crystallinity, and increased swelling with increasing drug content of 1, 2, and 4 wt %. Additionally, increasing the SN-38 concentration reduced the degradation rate of the GT. Furthermore, we hypothesize the existence of a drug content saturation point in the monoaxial fiber to explain the different drug release patterns of PG2 compared with those of PG1 and PG4. The matrix also showed good biodegradation and anti-tumor function. Our results demonstrate that SN-38-loaded PCL/GT fibers can be obtained by electrospinning. The SN-38-loaded fibers merit further evaluation as a means to potentially prevent locoregional recurrence following surgical tumor resection.