Phylogenetic diversity of the picocyanobacterial community from a novel winter bloom in Lake Biwa
Phylogenetic diversity of the picocyanobacterial community from a novel winter bloom in Lake Biwa
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DOI：
10.1007/s10201-020-00649-5
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发表时间：
2021-02-04
期刊：
影响因子：
1.6
通讯作者：
Nakano, Shin-ichi
中科院分区：
文献类型：
--
作者：
Cai, Ji;Hodoki, Yoshikuni;Nakano, Shin-ichi
在琵琶湖，picocyanobacteria水华通常发生在夏秋季节的温度分层期间。有趣的是，在2015-2016年冬季发现了一种新的水华，尽管水温较低，但微蓝细菌细胞密度增加了一个数量级，这表明“冷水偏好”物种在微蓝细菌群落中占主导地位的可能性。本研究通过分析16 S rRNA基因，探讨了日本琵琶湖微蓝细菌的系统发育多样性。我们发现，在琵琶湖的picocyanobacteria社区是高度多样化的，与8集球藻相关的操作分类单位（OTUs）在不同的季节检测。这些OTU属于不同的系统发育组，大多数与以前报道的集群密切相关。值得注意的是，在冬季水华期间检测到的OTU 04与在各种湖泊中发现的序列高度相关，例如西藏湖泊和上级湖，其中水体通常具有低营养状态和温度，以及不同浓度的总溶解固体。因此，我们推断，组包含OTU 04可能是一个psychrotolerant谱系，广泛分布在贫营养水系统中的低-中等盐度。
In Lake Biwa, picocyanobacteria blooms usually occur during the summer-autumn thermal stratification period. Intriguingly, a novel bloom was detected in winter 2015-2016, in which picocyanobacterial cell density increased by one order of magnitude despite lower water temperature, suggesting the possibility that "cold-water-preference" species dominate in the picocyanobacterial community. In the present study, we investigated the phylogenetic diversity of picocyanobacteria in Lake Biwa by analyzing the 16S rRNA gene. We found that the picocyanobacterial community were highly diverse in Lake Biwa, with eight Synechococcus-related operational taxonomic units (OTUs) detected in different seasons. These OTUs fell into distinct phylogenetic groups, and the majority were closely related to clusters reported previously. Notably, OTU04, detected during the winter bloom, was highly affiliated with sequences found in a variety of lakes, such as Tibetan lakes and Lake Superior, where the water bodies generally have a low trophic state and temperature, and different concentrations of total dissolved solids. Thus, we inferred that the group containing OTU04 may be a psychrotolerant lineage that is widely distributed in oligotrophic water systems with low-intermediate salinity.