Metal enhanced fluorescence improved protein and DNA detection by zigzag Ag nanorod arrays
Metal enhanced fluorescence improved protein and DNA detection by zigzag Ag nanorod arrays
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金属增强荧光改善了锯齿形银纳米棒阵列的蛋白质和 DNA 检测
DOI：
10.1016/j.bios.2016.04.022
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发表时间：
2016-08-15
影响因子：
12.6
通讯作者：
Fu, Junxue
中科院分区：
文献类型：
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作者：
Ji, Xiaofan;Xiao, Chenyu;Fu, Junxue
由于金属纳米阵列比随机纳米结构在金属增强荧光（MEF）方面表现出巨大的潜力，因此通过斜角沉积制备的银锯齿形纳米棒（ZNR）阵列的MEF已被研究用于生物分子-蛋白质相互作用和DNA杂交。通过改变折叠数和沉积基底温度，可以获得14倍的生物素-中性亲和素检测增强因子(EF)。由于Ag ZNRs 的高散射强度，最佳折叠数确定为Z=7。基底温度T=25℃和0℃稍微改变了Ag ZNR的形貌，但EF没有太大差异。此外，沉积在一层Ag薄膜上的Ag ZNR已被引入DNA杂交中，并且通过荧光显微镜观察到显着的信号增强。通过详细的定量 EF 分析，排除了 ZNR 表面积增加的增强效应，仅考虑 MEF 的贡献，两条 33 个碱基的单链寡核苷酸杂交的 EF 为 28。此外，检测限确定为0.01 pM。我们相信 Ag ZNR 阵列可以作为通用且灵敏的生物检测平台。 (C) 2016 Elsevier B.V. 保留所有权利。
As metal nano-arrays show great potential on metal enhanced fluorescence (MEF) than random nanostructures, MEF of Ag zigzag nanorod (ZNR) arrays made by oblique angle deposition has been studied for biomolecule-protein interaction and DNA hybridization. By changing the folding number and the deposition substrate temperature, a 14-fold enhancement factor (EF) is obtained for biotin-neutravidin detection. The optimal folding number is decided as Z=7, owing to the high scattering intensity of Ag ZNRs. The substrate temperature T=25 degrees C and 0 degrees C slightly alters the morphology of Ag ZNRs but has no big difference in EF. Further, Ag ZNRs deposited on a layer of Ag film have been introduced to the DNA hybridization and a significant signal enhancement has been observed through the fluorescence microscope. Through a detailed quantitative EF analysis, which excludes the enhancing effect from the increased surface area of ZNRs and only considers the contribution of MEF, an EF of 28 is achieved for the hybridization of two single-stranded oligonucleotides with 33 bases. Furthermore, a limit of detection is determined as 0.01 pM. We believe that the Ag ZNR arrays can serve as a universal and sensitive biodetection platform. (C) 2016 Elsevier B.V. All rights reserved.