Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China
Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China
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DOI：
10.3347/kjp.2019.57.1.153
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发表时间：
2019-04-01
影响因子：
--
通讯作者：
Jia, Bin
中科院分区：
文献类型：
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作者：
Guo, Baoping;Zhang, Zhuangzhi;Jia, Bin
细粒棘球绦虫是一种重要的人畜共患寄生虫，在全球范围内引起人类和动物的囊性包虫病（CE）。本研究对从中国北疆不同地区收集的细粒棘球绦虫的CE流行情况和cox1基因序列变异进行了分析。调查显示，3.5%的羊和4.1%的牛感染了CE。通过PCR从所有阳性羊和牛样本中扩增出cox1片段。此外，还包括4个地区的26个阳性样本。这些分离株均为细粒细粒棘球菌，含有 15 个单倍型 (Hap1-15)，并聚类为 2 个基因型：G1 (90.1%, 91/101) 和 G3 (9.9%, 10/101)。 Hap1 是最常见的单倍型 (48.5%, 49/101)。 Hap9在人类样本中被发现，表明羊和牛是人类CE的宿主。这表明细粒棘球蚴可能对该地区牲畜和人类的CE控制产生影响。
Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in humans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.