Micro-CT and histological investigation of the spatial pattern of feto-placental vascular density
Micro-CT and histological investigation of the spatial pattern of feto-placental vascular density
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DOI：
10.1016/j.placenta.2019.09.014
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发表时间：
2019-12-01
期刊：
影响因子：
3.8
通讯作者：
David, A. L.
中科院分区：
文献类型：
--
作者：
Aughwane, R.;Schaaf, C.;David, A. L.
引言：正常胎盘内绒毛形态有相当大的差异。然而，是否有一个可重复的绒毛血管密度的变化空间模式是未知的。Micro-CT提供空间分辨率低至微米级的三维体积成像。在这项研究中，我们应用Micro-CT和组织学分析，探讨胎盘内的血管化的异质性的程度。方法：10个长期胎盘收集在择期剖腹产，灌注造影剂和成像整体与Micro-CT。然后从每个胎盘中取出8个全深度组织块并成像。取切片进行组织学分析。数据进行了分析，调查血管填充，血管密度的位置从脐带插入胎盘边缘在每个scale.Results：整个胎盘成像显示没有空间一致的差异，绒毛血管密度的主要胎盘组织内，虽然有很大程度的异质性。组织块成像和组织学分析均发现胎盘内血管密度的高度异质性，但绒毛血管密度和组织块位置之间没有强相关性（r（s）= 0.066，p = 0.7组织块成像，r（s）= 0.06，p = 0.6组织学分析）。本文提出了一种利用多尺度Micro-CT成像技术对胎盘血管树进行成像的新方法。它表明，在整个正常足月人胎盘中，血管密度存在很大程度的变化。通过这种技术创建的三维数据可以用于更先进的计算机分析，以进一步研究血管树的结构。
Introduction: There are considerable variations in villous morphology within a normal placenta. However, whether there is a reproducible spatial pattern of variation in villous vascular density is not known. Micro-CT provides three-dimensional volume imaging with spatial resolution down to the micrometre scale. In this study, we applied Micro-CT and histological analysis to investigate the degree of heterogeneity of vascularisation within the placenta.Method: Ten term placentas were collected at elective caesarean section, perfused with contrast agent and imaged whole with Micro-CT. Eight full depth tissue blocks were then taken from each placenta and imaged. Sections were taken for histological analysis. Data was analysed to investigate vascular fill, and vascular density in relation to location from cord insertion to placental edge at each scale.Results: Whole placental imaging revealed no spatially consistent difference in villous vessel density within the main placental tissue, although there was a great degree of heterogeneity. Both block imaging and histological analysis found a large degree of heterogeneity of vascular density within placentas, but no strong correlation between villous vascular density and block location (r(s) = 0.066, p = 0.7 block imaging, r(s) = 0.06, p = 0.6 histological analysis).Discussion: This work presents a novel method for imaging the human placenta vascular tree using multiscale Micro-CT imaging. It demonstrates that there is a large degree of variation in vascular density throughout normal term human placentas. The three-dimensional data created by this technique could be used, with more advanced computer analysis, to further investigate the structure of the vascular tree.