AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis.
AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis.
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Atalmt9是拟南芥中气孔开口所需的苹果酸液泡氯化物通道。
DOI：
10.1038/ncomms2815
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发表时间：
2013
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
水分亏缺严重影响作物生产力。植物通过调节叶表皮气孔的开度来控制水分流失和CO2吸收。气孔开度由两个保卫细胞调节，保卫细胞形成气孔并响应离子的吸收和释放而改变其大小。虽然我们的知识钾和氯通量通过质膜的保卫细胞是先进的，很少有人知道通量通过液泡膜。在这里，我们提出了长期寻求后的液泡氯离子通道的分子鉴定。AtALMT9是由生理浓度的细胞溶质苹果酸激活的氯离子通道。单通道测量表明，这种激活是由于苹果酸依赖的通道开放概率增加。拟南芥atalmt9基因敲除突变体表现出受损的气孔开放和枯萎比野生型更慢。我们的研究结果表明，AtALMT9是一个液泡氯离子通道具有控制气孔孔径的主要作用。 苹果酸转运蛋白是植物独有的，调节离子穿过其所表达的膜的转运。De Angeli及其同事表明，AtALMT9作为一种液泡氯离子通道，由胞质苹果酸激活，并调节气孔孔径。
Water deficit strongly affects crop productivity. Plants control water loss and CO2 uptake by regulating the aperture of the stomatal pores within the leaf epidermis. Stomata aperture is regulated by the two guard cells forming the pore and changing their size in response to ion uptake and release. While our knowledge about potassium and chloride fluxes across the plasma membrane of guard cells is advanced, little is known about fluxes across the vacuolar membrane. Here we present the molecular identification of the long-sought-after vacuolar chloride channel. AtALMT9 is a chloride channel activated by physiological concentrations of cytosolic malate. Single-channel measurements demonstrate that this activation is due to a malate-dependent increase in the channel open probability. Arabidopsis thaliana atalmt9 knockout mutants exhibited impaired stomatal opening and wilt more slowly than the wild type. Our findings show that AtALMT9 is a vacuolar chloride channel having a major role in controlling stomata aperture. Aluminium-activated malate transporters are exclusive to plants, regulating the transport of ions across the membranes on which they are expressed. De Angeli and colleagues show that AtALMT9 acts as a vacuolar chloride channel that is activated by cytosolic malate, and that this regulates stomata aperture.
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影响因子：
7.2
作者：
Kovermann, Peter;Meyer, Stefan;Martinoia, Enrico
通讯作者：
Martinoia, Enrico
影响因子：
7.4
作者：
Talbott, LD;Zeiger, E
通讯作者：
Zeiger, E
影响因子：
11.4
作者：
Pei, ZM;Ward, JM;Schroeder, JI
通讯作者：
Schroeder, JI
DOI：
10.1073/pnas.0602868103
发表时间：
2006-06-20
影响因子：
11.1
作者：
Hoekenga, Owen A.;Maron, Lyza G.;Kochian, Leon V.
通讯作者：
Kochian, Leon V.
影响因子：
7.4
作者：
GERHARDT, R;HELDT, HW
通讯作者：
HELDT, HW