Bisphenol S, a Bisphenol A alternative, impairs swine ovarian and adipose cell functions
Bisphenol S, a Bisphenol A alternative, impairs swine ovarian and adipose cell functions
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DOI：
10.1016/j.domaniend.2018.08.001
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发表时间：
2019-01-01
影响因子：
2.1
通讯作者：
Basini, G.
中科院分区：
文献类型：
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作者：
Berni, M.;Gigante, P.;Basini, G.
大量生产的塑料单体双酚A（BPA）由于其内分泌干扰物（艾德）行为，在过去几年中一直备受关注，导致人类和野生动物广泛暴露于BPA与生殖，代谢和发育障碍以及依赖性癌症发病之间的关联被披露。这些证据导致限制和禁止BPA的工业用途，并促使无害的替代化合物的调查。最重要的是，一些国家已经在婴儿护理产品制造中用双酚S（BPS）替代了母体类似物，即使其与BPA的结构同源性表明类似的艾德特性尚未完全排除。鉴于这一考虑，本体外研究的目的是研究BPS暴露（0.1、1和10 μ M，持续48 h）对颗粒细胞的影响，颗粒细胞被认为是BPA作为“生殖毒物”的主要卵巢靶点。我们的数据表明，BPS抑制E2的产生，细胞增殖和清除非酶活性（P < 0.05），而它显着（P < 0.05）刺激细胞活力，超氧阴离子（O-2（-））和一氧化氮（NO）的生产在培养的猪颗粒细胞，以前验证的内分泌细胞模型BPA。还有证据表明，BPA及其类似物作为环境亲脂性污染物，参与破坏脂肪组织（AT）内分泌功能，导致代谢效应，从而导致潜在的生殖障碍。在此基础上，我们的第二个目的是评估BPS对从猪AT分离的间充质基质细胞（MSC）的影响，考虑到MSC的活力和成脂分化，这一过程实际上被证明在很大程度上受到ED的影响。我们的研究结果表明，BPS降低（P < 0.001）增殖的脂肪基质细胞的细胞活力。作为一个整体，我们的数据表明，有效的BPS艾德活性在μ M浓度，这表明，在考虑将其用于工业应用作为BPA的替代品之前，需要进一步的研究。(C)2018爱思唯尔公司All rights reserved.
The high-volume-produced plastic monomer Bisphenol A (BPA) has been in the spotlight in the last years because of its endocrine disruptor (ED) behavior, leading to disclosure of the association between the widespread human and wildlife exposure to BPA and reproductive, metabolic, and developmental disorders and hormone-dependent cancer onset. These evidences caused restrictions and prohibitions of BPA industrial uses and prompted investigation of harmless alternative compounds. Above all, several countries have substituted the parental analogue with Bisphenol S (BPS) in baby care product manufacturing, even if its structural homology to BPA suggests similar ED properties not yet completely ruled out. In light of this consideration, the aim of this in vitro study was to investigate the effect of BPS exposure (0.1, 1, and 10 mu M for 48 h) on granulosa cells that are considered the prime ovarian targets of BPA as a "reproductive toxicant". Our data document that BPS inhibited E2 production, cell proliferation, and scavenging nonenzymatic activity (P < 0.05) while it significantly (P < 0.05) stimulated cell viability, superoxide (O-2(-)) and nitric oxide (NO) production in cultured swine granulosa cells, a previously validated endocrine cell model for BPA. Evidence also exists that BPA and its analogues, as environmental lipophilic pollutants, are involved in the disruption of adipose tissue (AT) endocrine function, resulting in metabolic effects and thus in potential reproductive disorders. On this basis, our second purpose was the assessment of BPS effects on mesenchymal stromal cells (MSCs) isolated from porcine AT, taking into account MSCs viability and adipogenic differentiation, a process actually demonstrated to be largely affected by EDs. Our results show that BPS decreased (P < 0.001) cell viability of proliferating adipose stromal cells. Taken as a whole, our data demonstrate an effective BPS ED activity at mu M concentrations, suggesting that further studies are needed before considering its use in industrial application as an alternative to BPA. (C) 2018 Elsevier Inc. All rights reserved.