The Influence of Vegetation Type on the Dominant Soil Bacteria, Archaea, and Fungi in a Low Arctic Tundra Landscape
The Influence of Vegetation Type on the Dominant Soil Bacteria, Archaea, and Fungi in a Low Arctic Tundra Landscape
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DOI：
10.2136/sssaj2011.0057
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发表时间：
2011-09-01
影响因子：
2.9
通讯作者：
Grogan, Paul
中科院分区：
文献类型：
--
作者：
Chu, Haiyan;Neufeld, Josh D.;Grogan, Paul
由于地形和水文条件的景观异质性，北极植被群落在短距离内变化很大，但土壤微生物群落的相应模式和控制还不清楚。我们的特点和比较最丰富的重复土壤微生物群落（n = 4）的四个主要的植被类型在加拿大低北极苔原（干石南，桦树丘，高桦树，湿莎草）内的微生物类型，使用变性梯度凝胶电泳（DGGE）的小亚基rRNA基因。我们确定了10种主要的细菌类型。虽然大多数都存在于所有的土壤样品中，其相对丰度差异显着，并根据植被类型一致。与此相反，所有植被类型的真菌群落占主导地位的两个常见的真菌类型。的主要古菌（11个确定）的社区之间的一些植被类型，甚至在相同的植被类型的复制补丁之间的差异很大，这表明大的空间异质性，不能归因于植被类型的影响。所有植被类型中的细菌和真菌群落分别以酸杆菌和接合菌门为主。高桦树和湿莎草中Euryarchaeota占主导地位，虽然Euryarchaeota和Thaumarchaeota丰富的桦树丘和干燥的石南土壤。我们的结论是，植被类型对土壤细菌群落结构产生了强烈的影响，并在低北极苔原古菌和真菌群落的影响相对较小，变化。最后，在植被类型之间的细菌群落结构的变化与土壤可溶性N和N矿化潜力，表明优势土壤细菌的相对丰度和N的可用性之间的密切联系在整个低北极苔原。
Arctic vegetation communities vary greatly over short distances due to landscape heterogeneities in topography and hydrological conditions, but corresponding patterns and controls for soil microbial communities are not well understood. We characterized and compared the most abundant phylotypes within replicate soil microbial communities (n = 4) underlying the four principal vegetation types in Canadian low Arctic tundra (dry heath, birch hummock, tall birch, and wet sedge) using denaturing gradient gel electrophoresis (DGGE) of small subunit rRNA genes. We identified 10 major bacterial phylotypes. Although most were present in all soil samples, their relative abundances differed significantly and consistently according to vegetation type. By contrast, the fungal communities of all vegetation types were dominated by two common phylotypes. The communities of major archaea (11 identified) differed substantially among some of the vegetation types and even among replicate patches of the same vegetation type, indicating large spatial heterogeneities that could not be attributed to the influence of vegetation type. Bacterial and fungal communities in all vegetation types were dominated by Acidobacteria and Zygomycota, respectively. Archaeal communities were dominated by Euryarchaeota in tall birch and wet sedge although both Euryarchaeota and Thaumarchaeota were abundant in the birch hummock and dry heath soils. We conclude that vegetation type exerts a strong influence on soil bacterial community structure, and a relatively small and varying influence on archaeal and fungal communities in low Arctic tundra. Finally, variation in bacterial community structure among the vegetation types was correlated with soil soluble N and N mineralization potential, suggesting a close association between the relative abundances of dominant soil bacteria and N availability across low Arctic tundra.