CaO2/gelatin oxygen slow-releasing microspheres facilitate tissue engineering efficiency for the osteonecrosis of femoral head by enhancing the angiogenesis and survival of grafted bone marrow mesenchymal stem cells.
CaO2/gelatin oxygen slow-releasing microspheres facilitate tissue engineering efficiency for the osteonecrosis of femoral head by enhancing the angiogenesis and survival of grafted bone marrow mesenchymal stem cells.
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DOI：
10.1039/d0bm02071k
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发表时间：
2021-03
影响因子：
6.6
通讯作者：
Chengqiang Wang;Haixia Xu;Chun Liu;Ziyue Peng;Ruoxing Min;Zhiming Zhang;Jianjun Li;Yanglei Jin;Yihan Wang;Zhihao Li;Jiasong Guo;Lixin Zhu
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文献类型：
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作者：
Chengqiang Wang;Haixia Xu;Chun Liu;Ziyue Peng;Ruoxing Min;Zhiming Zhang;Jianjun Li;Yanglei Jin;Yihan Wang;Zhihao Li;Jiasong Guo;Lixin Zhu
股骨头坏死（ONFH）是一种常见的难治性疾病，至今人们仍未完全了解。缺血引起的缺氧不仅是ONFH的重要致病因素，也是组织工程治疗ONFH中种子细胞存活的关键挑战。为了探索针对缺氧治疗 ONFH 的有效策略，新设计的 CaO2/明胶微球与 3D 打印聚己内酯/纳米羟基磷灰石（PCL/nHA）多孔支架、海藻酸钠/明胶水凝胶和骨髓间充质干细胞（BMSC）复合，开发出一种新型组织工程支架，然后移植到 ONFH 兔模型的核心凹陷区域。目前的数据表明，CaO2/明胶微球可以持续释放氧气19天。 BMSCs 体外实验表明支架具有较高的生物相容性，有利于极度缺氧（1% O2）下的细胞增殖。体内研究表明，与不含微球的支架相比，带有产氧微球的移植支架显着增强了成骨和血管生成作用。进一步的评估表明，支架中的微球可以减少局部细胞凋亡，提高移植细胞在宿主体内的存活率。总的来说，本研究开发了一种新型氧气缓释复合支架，它可以通过增强血管生成和移植干细胞的存活来提高治疗股骨头坏死的组织工程效率。
The osteonecrosis of femoral head (ONFH), a common refractory disease, is still not fully understood today. Hypoxia caused by ischemia is not only an important pathogenic factor but also a critical challenge for the survival of seed cells in the tissue engineering therapy of ONFH. To explore an efficient strategy to treat ONFH by targeting hypoxia, newly designed CaO2/gelatin microspheres were composited with 3D printed polycaprolactone/nano-hydroxyapatite (PCL/nHA) porous scaffold, sodium alginate/gelatin hydrogel, and bone marrow mesenchymal stem cells (BMSCs) to develop a novel tissue engineering scaffold and then transplanted into the core depression area of the ONFH rabbit model. The current data demonstrated that CaO2/gelatin microspheres can constantly release oxygen for 19 days. In vitro assays with BMSCs illustrated that scaffolds have high biocompatibility and are favorable for cell proliferation in extreme hypoxia (1% O2). The in vivo study demonstrated that the transplanted scaffold with oxygen-generating microspheres significantly enhanced the osteogenic and angiogenic effects compared to the scaffold without microspheres. Further assessments revealed that microspheres in the scaffold can reduce the local cell apoptosis and enhance the survival of grafted cells in the host. Collectively, the present study developed a novel oxygen slow-releasing composite scaffold, which can facilitate tissue engineering efficiency for treating the osteonecrosis of the femoral head by enhancing the angiogenesis and survival of grafted stem cells.