Rapid decreases in preoptic aromatase activity and brain monoamine concentrations after engaging in male sexual behavior
Rapid decreases in preoptic aromatase activity and brain monoamine concentrations after engaging in male sexual behavior
复制标题
DOI：
10.1210/en.2005-0441
复制
发表时间：
2005-09-01
期刊：
影响因子：
4.8
通讯作者：
Balthazart, J
中科院分区：
文献类型：
--
作者：
Cornil, CA;Dalla, C;Balthazart, J
日本鹌鹑和老鼠一样，在相对较长的时间内（几天到几周），雄性性行为的表达依赖于视前区通过睾酮的芳香化产生的雌二醇。在短期内（几分钟到几小时），多巴胺的中枢活动以及局部产生的雌激素调节行为表达。在大鼠中，看到雌性和与雌性发生性行为会增加视前区多巴胺的释放。在鹌鹑中，体外脑芳香酶活性（AA）被钙依赖性磷酸化快速调节，这种磷酸化可能由于神经递质活性的变化而在体内发生。此外，急性雌二醇注射会迅速刺激鹌鹑的交配，而单次注射芳香酶抑制剂vorozole会迅速抑制这种行为。我们假设鹌鹑的脑芳香化酶和多巴胺能活性与雄性性行为的表达有关。视觉接触和与女性的性接触均显著降低了脑AA，在5分钟后达到最大。这种性行为的表达也导致1分钟后多巴胺能和5 -羟色胺能活性显著下降，5分钟后恢复到基础水平。这些结果首次证明了AA在体内与多巴胺活性的变化并行快速调节。与雌性发生性行为会降低芳香化酶和多巴胺的活性。这些数据挑战了关于多巴胺、雌激素作用和男性性行为之间因果关系的既定观点。
In Japanese quail, as in rats, the expression of male sexual behavior over relatively long time periods (days to weeks) is dependent on the local production of estradiol in the preoptic area via the aromatization of testosterone. On a short-term basis (minutes to hours), central actions of dopamine as well as locally produced estrogens modulate behavioral expression. In rats, a view of and sexual interaction with a female increase dopamine release in the preoptic area. In quail, in vitro brain aromatase activity (AA) is rapidly modulated by calcium-dependent phosphorylations that are likely to occur in vivo as a result of changes in neurotransmitter activity. Furthermore, an acute estradiol injection rapidly stimulates copulation in quail, whereas a single injection of the aromatase inhibitor vorozole rapidly inhibits this behavior. We hypothesized that brain aromatase and dopaminergic activities are regulated in quail in association with the expression of male sexual behavior. Visual access as well as sexual interactions with a female produced a significant decrease in brain AA, which was maximal after 5 min. This expression of sexual behavior also resulted in a significant decrease in dopaminergic as well as serotonergic activity after 1 min, which returned to basal levels after 5 min. These results demonstrate for the first time that AA is rapidly modulated in vivo in parallel with changes in dopamine activity. Sexual interactions with the female decreased aromatase and dopamine activities. These data challenge established views about the causal relationships among dopamine, estrogen action, and male sexual behavior.