Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart.
Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart.
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DOI：
10.3389/fphar.2017.00312
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发表时间：
2017
影响因子：
5.6
通讯作者：
Rungatscher A
中科院分区：
文献类型：
--
作者：
Ahmed N;Linardi D;Decimo I;Mehboob R;Gebrie MA;Innamorati G;Luciani GB;Faggian G;Rungatscher A
目的：1-磷酸鞘氨醇 (S1P) 鞘脂衍生物是已知的抗炎、抗凋亡和抗氧化剂。 S1P 已被证明在心血管系统中发挥作用。本研究的目的是了解S1P受体（S1PR）在人和大鼠心血管组织中的精确表达和分布，以了解我们在大鼠模型中的实验研究的意义和可能的实施。方法和结果：在本研究中，我们研究了维罗纳大学医院心脏外科部门的人类心脏样本和大鼠样本中 S1PR 的定位。石蜡包埋切片的免疫组织化学研究表明，人和大鼠的心肌样本存在弥漫性染色。人心脏的信号与大鼠心脏所有心室的信号相似。免疫组织化学表达水平与基于 RT-PCR 的分析和蛋白质印迹的结果密切相关。我们通过各种技术证实S1PR1表达强于S1PR3，并且均匀分布在心脏的所有腔室中，在人和大鼠心肌组织中没有显着差异。在人和大鼠心脏的 RT-PCR 结果中，S1PR2 表达显着较弱，而 S1PR4 和 S1PR5 未检测到。结论：这些结果表明，在大鼠模型上使用S1PR激动剂的实验研究更有可能转化为临床研究，本研究揭示的第二个重要信息是，S1P受体激动剂可用于全身缺血再灌注损伤的心脏保护。
Aim: Sphingosine 1-phosphate (S1P), sphingolipid derivatives are known anti-inflammatory, anti-apoptotic, and anti-oxidant agent. S1P have been demonstrated to have a role in the cardiovascular system. The purpose of this study was to understand the precise expression and distribution of S1P receptors (S1PRs) in human and rat cardiovascular tissues to know the significance and possible implementation of our experimental studies in rat models. Methods and Results: In this study, we investigated the localization of S1PRs in human heart samples from cardiac surgery department, University of Verona Hospital and rat samples. Immunohistochemical investigation of paraffin-embedded sections illustrated diffused staining of the myocardial samples from human and rat. The signals of the human heart were similar to those of the rat heart in all chambers of the heart. The immunohistochemical expression levels correlated well with the results of RT-PCR-based analysis and western blotting. We confirmed by all techniques that S1PR1 expressed strongly as compared to S1PR3, and are uniformly distributed in all chambers of the heart with no significant difference in human and rat myocardial tissue. S1PR2 expression was significantly weak while S1PR4 and S1PR5 were not detectable in RT-PCR results in both human and rat heart. Conclusion: These results indicate that experimental studies using S1PR agonists on rat models are more likely to have a potential for translation into clinical studies, and second important information revealed by this study is, S1P receptor agonist can be used for cardioprotection in global ischemia-reperfusion injury.