Serological identification and insect toxicity of Bacillus thuringiensis isolated from the island Okinoerabu-jima, Japan
Serological identification and insect toxicity of Bacillus thuringiensis isolated from the island Okinoerabu-jima, Japan
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日本冲良部岛苏云金芽孢杆菌的血清学鉴定及昆虫毒性
DOI：
10.1303/aez.2007.285
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发表时间：
2007
影响因子：
1.3
通讯作者：
M. Ohba
中科院分区：
文献类型：
--
作者：
Koichi Yasutake;Akiko Uemori;Kumiko Kagoshima;M. Ohba
我们检查了日本鹿儿岛县冲良部岛的土壤中苏云金芽孢杆菌的发生情况。在20个土壤采样点中有13个（65%），在100个土壤样品中有27个（27%）。在检查的2，680个蜡样芽孢杆菌群菌落中，66个被分配到B。苏云金杆菌岛上的土壤提供了至少11种H-血清型。大多数菌株没有表现出杀虫活性，而一个分离的serovar kurstaki（H3 abc）与双锥体伴孢内含物杀死双翅目和鳞翅目幼虫，和另一个分离的serovar colmeri（H21）与不规则尖的内含物有很高的杀蚊活性。7株以色列血清变型菌株（H14）均未表现出杀蚊幼虫活性，但产生的伴孢内含物形态与具有强杀蚊活性的以色列血清变型参考菌株相似。
We examined soils of the island Okinoerabu-jima, Kagoshima Prefecture, Japan, for the occurrence of Bacillus thuringiensis. The organism occurred in 13 (65%) out of 20 soil-sampling sites, and 27 (27%) out of 100 soil samples. Among 2,680 Bacillus cereus group colonies examined, 66 were allocated to B. thuringiensis. Soils of the island provided at least 11 H-serotypes. Most of the isolates exhibited no insecticidal activities, while an isolate of serovar kurstaki (H3abc) with bipyramidal parasporal inclusions killed both dipteran and lepidopteran larvae, and another isolate of serovar colmeri (H21) with irregularly-pointed inclusions had a high mosquitocidal activity. None of the seven isolates of serovar israelensis (H14) showed mosquito larvicidal activities, while producing parasporal inclusions morphologically similar to those of the reference strain of serovar israelensis with strong mosquitocidal activities.