Oligoclonal bands and immunoglobulin isotype switch during monitoring of patients with multiple myeloma and autologous hematopoietic cell transplantation: a 16-year experience
Oligoclonal bands and immunoglobulin isotype switch during monitoring of patients with multiple myeloma and autologous hematopoietic cell transplantation: a 16-year experience
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DOI：
10.1515/cclm.2010.050
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发表时间：
2010-05-01
影响因子：
6.8
通讯作者：
Antonio Pizzolato, Marco
中科院分区：
文献类型：
--
作者：
Emilce Alejandre, Mariel;Bibiana Madalena, Leticia;Antonio Pizzolato, Marco
背景资料：在过去的20年里，高剂量治疗后的造血干细胞移植已被用于多发性骨髓瘤（MM）患者。方法：采用琼脂糖凝胶电泳结合光密度分析和免疫固定技术，对238例自体造血干细胞移植（ASCT）患者的血清单克隆蛋白进行分析。定量IgM、伊加、IgG和β 2-微球蛋白（β 2 M）。尿蛋白电泳与IF进行醋酸纤维素凝胶上使用胶体银染色不浓缩。结果：经过34个月的随访（范围1-160个月），8例（3.4%）显示出一个独特的单克隆蛋白带，是从他们原来的同种型开关不同。观察到这是一种短暂现象（22.2个月）。37例患者（15.5%）在ASCT后1 - 20个月（平均4.4个月）出现寡克隆带（OB），持续时间为7.9个月（1-36个月）。平均总生存时间差异有统计学意义（p
Background: Over the last 20 years, high dose therapy followed by hemopoietic stem cell transplantation has been employed in patients with multiple myeloma (MM). During 16 years of follow-up, the degree of tumor response and survival in 238 patients with autologous stem cell transplantation (ASCT) and changes in the serum protein electrophoretic pattern were analyzed.Methods: Agarose gel electrophoresis with densitometric analysis and immunofixation were performed to evaluate serum monoclonal protein. IgM, IgA, IgG and beta(2)-microglobulin (beta 2M) were quantitated. Urine protein electrophoresis with IF was performed on cellulose acetate gel using colloidal silver staining without concentrating.Results: After 34 months of follow-up (range 1-160 months), eight patients (3.4%) showed a distinct monoclonal protein band that was different from their original isotype switch. This was observed to be a transient phenomenon (22.2 months). Thirty-seven patients (15.5%) developed oligoclonal bands (OB) between the first and the twentieth month after ASCT (mean 4.4 months), which persisted for 7.9 months (1-36 months). The mean overall survival time was statistically different (p