FLOW CYTOMETRIC MEASUREMENT OF NUCLEAR-DNA CONTENT VARIATIONS AS A POTENTIAL INVIVO MUTAGENICITY TEST
FLOW CYTOMETRIC MEASUREMENT OF NUCLEAR-DNA CONTENT VARIATIONS AS A POTENTIAL INVIVO MUTAGENICITY TEST
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DOI：
10.1002/cyto.990020311
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
JAIN, VK
中科院分区：
文献类型：
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作者：
OTTO, FJ;OLDIGES, H;JAIN, VK
采用高分辨率流式细胞术研究了致突变剂诱导的小鼠骨髓细胞DNA含量的变化。在单次注射化学诱变剂环磷酰胺后的第1天，观察到流量直方图G1峰的变异系数增加。该效应的幅度和持续时间具有剂量依赖性。测量的重现性很高，表明动物和仪器分散之间的个体差异很小。基于细胞DNA含量的流式细胞术测量，可以建立短期体内致突变性试验，其比常规细胞遗传学方法快得多。
Mutagen-induced variations of the cellular DNA content were studied in mouse bone marrow cells in vivo using high resolution flow cytometric techniques. During the 1st days after a single injection of the chemical mutagen cyclophosphamide an increased coefficient of variation in the G1 peak of the flow histograms was observed. The magnitude and duration of this effect were dose-dependent. The reproducibility of the measurements was high, indicating that individual variability between animals and instrumental dispersion is small. On the basis of the flow cytometric measurement of cellular DNA content, a short-term in vivo test for mutagenicity can be established which is much faster than conventional cytogenetic methods.