Effect of normobaric hyperoxia on two indexes of synaptic function in Fisher 344 rats
Effect of normobaric hyperoxia on two indexes of synaptic function in Fisher 344 rats
复制标题
DOI：
10.1016/s0891-5849(98)00260-3
复制
发表时间：
1999-04-01
影响因子：
7.4
通讯作者：
Joseph, J
中科院分区：
文献类型：
--
作者：
Bickford, PC;Chadman, K;Joseph, J
使用高氧大鼠模型研究了两种中枢神经系统神经递质受体对氧化应激的生理反应。我们发现，高氧会导致异丙肾上腺素 (ISO) 增强体内小脑浦肯野神经元 GABA 反应的能力下降。 N-叔丁基-α-苯基硝酮 (PBN) 可逆转这种效应。我们还表明，高氧会导致氧化震颤素（OXO）刺激纹状体切片中多巴胺（DA）释放的能力下降。这种效应伴随着中枢神经系统中羟自由基水平的增加，反映在 2,3-DHBA 的增加上，表明这种变化是氧化应激水平增加的结果。我们还显示高氧对β-肾上腺素能和毒蕈碱受体功能的时间依赖性影响。我们检查了年龄和高氧暴露之间的相互作用，发现 12 个月大的大鼠在暴露于氧气之前基线反应有所下降，这可能会干扰观察高氧的后续影响。在年龄和氧气暴露时间的相互作用方面，小脑和纹状体之间观察到了不同的影响。总体而言，数据表明年龄和高氧可能通过共同机制发挥作用，因为这两种情况没有协同作用。 (C) 1999 爱思唯尔科学公司。
The physiological response of two central nervous system neurotransmitter receptors to oxidative stress was studied using the rat model of hyperoxia. We show that hyperoxia leads to a decline in the ability of isoproterenol (ISO) to augment GABAergic responses in cerebellar Purkinje neurons in vivo. This effect is reversed by the N-tert-butyl-alpha-phenylnitrone (PBN). We also show that hyperoxia produces a decline in the ability of oxotremorine (OXO) to stimulate dopamine (DA) release in striatal slices. This effect is accompanied by an increase in hydroxyl radical levels in the CNS reflected in an increase in 2,3-DHBA, suggesting that the change is the result of an increased level of oxidative stress. We also show a time dependent effect of hyperoxia on both beta-adrenergic and muscarinic receptor function. We examined the interaction between age and hyperoxia exposure and found that in 12-month-old rats there is a decline in the baseline response prior to oxygen exposure that may interfere with observing a subsequent effect of hyperoxia. Differential effects were observed between the cerebellum and striatum with respect to the interaction of age and time of oxygen exposure. Overall, the data suggest that age and hyperoxia may be acting via a common mechanism because there was no synergistic effect of the two conditions. (C) 1999 Elsevier Science Inc.