Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis.
Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis.
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DOI：
10.1186/1748-717x-5-22
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发表时间：
2010-03-16
期刊：
影响因子：
--
通讯作者：
Keefe DM
中科院分区：
文献类型：
--
作者：
Ong ZY;Gibson RJ;Bowen JM;Stringer AM;Darby JM;Logan RM;Yeoh AS;Keefe DM
粘膜炎是抗癌治疗的毒副作用，是癌症研究的主要焦点。促炎细胞因子先前被认为与化疗引起的胃肠道粘膜炎的病理生理学有关。然而，它们是否在放疗引起的胃肠道粘膜炎的发展中发挥关键作用仍不清楚。因此，本研究的目的是使用分次放疗引起的毒性大鼠模型来表征胃肠道中促炎细胞因子的表达。 36 只雌性 Dark Agouti 大鼠被随机分组，并在六周内每周接受 3 次 2.5 Gys 腹部放射治疗。进行实时PCR以确定空肠和结肠中促炎细胞因子IL-1β、IL-6和TNF的mRNA表达的相对变化。使用定性免疫组织化学研究肠上皮中 IL-1β、IL-6 和 TNF 的蛋白表达。放疗引起的亚急性损伤与空肠和结肠中 IL-1β、IL-6 和 TNF mRNA 水平显着上调相关。分次放疗后，空肠和结肠中的大多数促炎细胞因子蛋白表达表现出最小的变化。促炎细胞因子在亚急性发作的放疗诱发的胃肠道粘膜炎中发挥关键作用。
Mucositis is a toxic side effect of anti-cancer treatments and is a major focus in cancer research. Pro-inflammatory cytokines have previously been implicated in the pathophysiology of chemotherapy-induced gastrointestinal mucositis. However, whether they play a key role in the development of radiotherapy-induced gastrointestinal mucositis is still unknown. Therefore, the aim of the present study was to characterise the expression of pro-inflammatory cytokines in the gastrointestinal tract using a rat model of fractionated radiotherapy-induced toxicity. Thirty six female Dark Agouti rats were randomly assigned into groups and received 2.5 Gys abdominal radiotherapy three times a week over six weeks. Real time PCR was conducted to determine the relative change in mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF in the jejunum and colon. Protein expression of IL-1β, IL-6 and TNF in the intestinal epithelium was investigated using qualitative immunohistochemistry. Radiotherapy-induced sub-acute damage was associated with significantly upregulated IL-1β, IL-6 and TNF mRNA levels in the jejunum and colon. The majority of pro-inflammatory cytokine protein expression in the jejunum and colon exhibited minimal change following fractionated radiotherapy. Pro-inflammatory cytokines play a key role in radiotherapy-induced gastrointestinal mucositis in the sub-acute onset setting.