Sensitive detection of multiple mycotoxins by SPRi with gold nanoparticles as signal amplification tags
Sensitive detection of multiple mycotoxins by SPRi with gold nanoparticles as signal amplification tags
复制标题
以金纳米粒子作为信号放大标签的 SPRi 灵敏检测多种霉菌毒素
DOI：
10.1016/j.jcis.2014.06.007
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发表时间：
2014-10-01
影响因子：
9.9
通讯作者：
Li, Chang Ming
中科院分区：
文献类型：
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作者：
Hu, Weihua;Chen, Hongming;Li, Chang Ming
多种有毒真菌毒素的检测在食品质量控制中具有重要意义。表面等离子体共振成像(SPRI)是一种同时进行多项准确检测的先进工具，但由于仪器的限制和真菌毒素的小尺寸，对于不敏感的竞争免疫分析，它的灵敏度有限。在这项工作中，金纳米颗粒(AuNP)增强的SPRI芯片被设计成使用竞争免疫分析的形式灵敏地检测多种真菌毒素。将致密的霉菌毒素抗原均匀地附着在聚[低聚(乙二醇甲基丙烯酸酯-甲基丙烯酸缩水甘油酯)](POEGMA-co-GMA)刷子修饰的SPRI金芯片上，构建了传感表面。在含有相应单抗的样品溶液中竞争性结合后，使用二次抗体偶联的AuNPs与捕获的单抗结合以进一步放大SPRI信号。对黄曲霉毒素B1(AFB1)、赭曲霉毒素A(OTA)和玉米赤霉烯酮(ZEN)三种典型真菌毒素实现了高特异性和高灵敏度的同时检测，检测下限分别为8、30和15pgmL(-1)，动态范围涵盖三个数量级。(C)2014 Elsevier Inc.保留所有权利。
Detection of multiple toxic mycotoxins is of importance in food quality control. Surface plasmon resonance imaging (SPRi) is an advanced tool for simultaneously multiple detections with accuracy; however, it suffers from limited sensitivity due to the instrumental constraint and small sizes of mycotoxins with only one epitope for an insensitive competitive immunoassay. In this work a gold nanoparticle (AuNP)-enhanced SPRi chip is.designed to sensitively detect multiple mycotoxins using a competitive immunoassay format. The sensing surface is constructed by uniformly attaching dense mycotoxin antigens on poly[oligo(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) brush modified SPRi gold chip. After competitive binding in a sample solution containing respective monoclonal antibodies, secondary antibody-conjugated AuNPs are employed to bind with the captured monoclonal antibodies for further amplification of the SPRi signal. Highly specific and sensitive simultaneous detection is achieved for three typical mycotoxins including Aflatoxin B1 (AFB1), Ochratoxin A (OTA) and Zearalenone (ZEN) with low detection limits of 8, 30 and 15 pg mL(-1) and dynamic ranges covering three orders of magnitude. (C) 2014 Elsevier Inc. All rights reserved.