INVIVO EFFECTS OF ENTERO-TOXIN FROM CLOSTRIDIUM-PERFRINGENS TYPE-A IN THE RABBIT COLON - BINDING VS BIOLOGIC ACTIVITY
INVIVO EFFECTS OF ENTERO-TOXIN FROM CLOSTRIDIUM-PERFRINGENS TYPE-A IN THE RABBIT COLON - BINDING VS BIOLOGIC ACTIVITY
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DOI：
10.1093/infdis/145.4.490
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发表时间：
1982-01-01
影响因子：
6.4
通讯作者：
DEMERS, GW
中科院分区：
文献类型：
--
作者：
MCDONEL, JL;DEMERS, GW
将纯化的A型产气荚膜梭菌肠毒素作用于兔结肠，分析肠毒素对结肠运输和表面结构的影响。与未经处理的动物相比，结肠在液体和电解质的运输中对肠毒素没有明显的反应。腔液蛋白水平显著增加，与粘液分泌增加相对应。组织学切片显示没有上皮损伤，但扫描电子显微镜显示粘液区的数量比对照动物的值增加。结肠对肠毒素治疗几乎没有明显的反应，但分离的结肠上皮细胞与125I标记的肠毒素的结合水平甚至高于回肠细胞中观察到的水平，回肠细胞中的肠毒素具有相当大的生物活性。因此，虽然肠毒素的结合对于其生物学活性是必要的，但生物学活性并不一定伴随着与特定受体的结合。
Rabbit colonic loops were exposed to purified enterotoxin from C. perfringens type A and perfused for analysis of the effects of the enterotoxin on transport and surface structure of the colon. The colon showed no apparent response to the enterotoxin in the transport of fluid and electrolytes as compared with untreated animals. There was a significant increase in levels of luminal fluid protein, corresponding to an observed increase in secretion of mucus. Histologic sections showed no epithelial damage, but scanning electron microscopy showed an increase in the number of mucous fields over the value in control animals. The colon showed little or no overt response to enterotoxin treatment, but isolated colonic epithelial cells bound 125I-labeled enterotoxin at levels even greater than those observed in cells from the ileum, where the enterotoxin has considerable biologic activity. Thus, although binding of enterotoxin is necessary for its biologic activity, biologic activity does not necessarily follow binding to specific receptors.