Spatial distribution of messenger RNA in the cytoskeletal framework of ascidian eggs.
Spatial distribution of messenger RNA in the cytoskeletal framework of ascidian eggs.
复制标题
海鞘卵细胞骨架框架中信使 RNA 的空间分布。
DOI：
10.1016/0012-1606(84)90335-x
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发表时间：
1984
影响因子：
2.7
通讯作者：
Jeffery,WR
中科院分区：
文献类型：
--
作者：
Jeffery,WR
母体poly（A）+RNA、组蛋白mRNA和肌动蛋白mRNA在海鞘卵的不同卵质区域表现出独特的空间分布。这些RNA似乎也迁移与他们各自的卵母细胞在广泛的细胞质重排发生受精后，这表明它们与结构框架。以TritonX-100抽提的皱瘤海鞘卵和早期胚胎为材料，用Poly（U）和克隆DNA探针进行原位杂交，研究了细胞骨架结构（CF）在决定母体mRNA空间分布中的作用。颗粒计数表明，相当大比例的卵poly（A）+RNA，组蛋白mRNA和肌动蛋白mRNA存在于CF中，并且在受精和两细胞阶段之间的期间，mRNA与CF相互作用的程度没有改变。颗粒分布的分析表明，聚（A）+RNA，组蛋白mRNA和肌动蛋白mRNA集中在相同的区域洗涤剂提取的鸡蛋，因为它们是在完整的鸡蛋。当细胞松弛素B或DNA酶I破坏肌动蛋白细胞骨架时，这些RNA在CF中的比例和空间分布不受影响。这些数据表明，母亲的mRNA与CF，这种协会是负责mRNA重排过程中卵质分离，和mRNA-CF的相互作用是不依赖于肌动蛋白细胞骨架的完整性。
Maternal poly(A)+RNA, histone mRNA, and actin mRNA exhibit unique spatial distributions in the different ooplasmic regions of ascidian eggs. These RNAs also appear to migrate with their respective ooplasms during the episode of extensive cytoplasmic rearrangement that occurs after fertilization, suggesting they are associated with a structural framework. The role of the cytoskeletal framework (CF) in determining the spatial distribution of maternal mRNA was tested by subjecting Triton X-100 extracted (Styela plicata) eggs and early embryos toin situhybridization with poly(U) and cloned DNA probes. Grain counts indicated that substantial proportions of the egg poly(A)+RNA, histone mRNA, and actin mRNA were present in the CF and that there was no alteration in the extent of mRNA-CF interactions during the period between fertilization and the two-cell stage. Analysis of grain distributions indicated that poly(A)+RNA, histone mRNA, and actin mRNA were concentrated in the same regions of detergent-extracted eggs as they are in intact eggs. The proportions and spatial distribution of these RNAs in the CF were not affected when the actin cytoskeleton was destabilized by cytochalasin B or DNAse I. The data suggest that maternal mRNA is associated with the CF, that this association is responsible for mRNA rearrangement during ooplasmic segregation, and that mRNA-CF interactions are not dependent on the integrity of the actin cytoskeleton.