Complex array of cytokines released by vasoactive intestinal peptide
Complex array of cytokines released by vasoactive intestinal peptide
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DOI：
10.1016/s0143-4179(03)00022-2
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发表时间：
2003-04-01
期刊：
影响因子：
2.9
通讯作者：
Gozes, I
中科院分区：
文献类型：
--
作者：
Brenneman, DE;Phillips, TM;Gozes, I
A complex mixture of five cytokines has been shown to be released by vasoactive intestinal peptide (VIP). Cytokines were measured in paired samples of culture medium and astroglial cytosol by capillary electrophoresis. This is the first description of VIP-mediated release for TNF-alpha, IL-3, G-CSF and M-CSF from astrocyte cultures. Kinetic studies after VIP treatment demonstrated a gradual but incomplete depletion of cytosolic cytokine levels, with differences observed among the cytokines. Significant increases in release were apparent within 15-30 min for all cytokines. As the recognized VIP receptors (VPAC1 and VPAC2) are linked to adenylate cyclase and also interact with pituitary adenylate cyclase activating polypeptide-38 (PACAP-38), both this homologous peptide and 8-bromo cAMP were investigated and compared to VIP-mediated release. Treatment with 1 mM 8-bromo cAMP produced cytokine release similar in amount to 0.1 nM PACAP-38, but significantly less (
