Characterization of Gene Expression Patterns among Artificially Developed Cancer Stem Cells Using Spherical Self-Organizing Map.
Characterization of Gene Expression Patterns among Artificially Developed Cancer Stem Cells Using Spherical Self-Organizing Map.
复制标题
使用球形自组织图的人为开发的癌症干细胞中基因表达模式的表征。
DOI：
10.4137/cin.s39839
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发表时间：
2016
影响因子：
2
通讯作者：
Seno M
中科院分区：
文献类型：
--
作者：
Seno A;Kasai T;Ikeda M;Vaidyanath A;Masuda J;Mizutani A;Murakami H;Ishikawa T;Seno M
我们对人工培养的肿瘤干细胞(CSCs)进行了基因表达微阵列分析和球面自组织映射(SSOM)。CSCs是从人诱导多能干细胞(HiPSCs)和癌细胞株的条件培养液中培养出来的，而CSCs是从人肿瘤组织的原代细胞培养中经特定因子(Oct3/4、SOX2和KLF4)诱导而来的。这些细胞通常表达人类胚胎干细胞(HESC)/hiPSC特异性基因(Pou5f1、SOX2、NANOG、LIN28和SALL4)，水平与对照HiPSC 201B7相当。非监督的SSOM方法表明，根据CSCs的培养条件和来源癌组织，CSCs可分为三组。此外，利用监督方法，SSOM提名了TMED9、RNAS1、NGFR、ST3GAL1、TNS4、BTG2、SLC16A3、CD177、CES1、GDF15、STMN2、FAM20A、NPPB、CD99、MYL7、PRSS23、AHNAK和LOC152573基因。
We performed gene expression microarray analysis coupled with spherical self-organizing map (sSOM) for artificially developed cancer stem cells (CSCs). The CSCs were developed from human induced pluripotent stem cells (hiPSCs) with the conditioned media of cancer cell lines, whereas the CSCs were induced from primary cell culture of human cancer tissues with defined factors (OCT3/4, SOX2, and KLF4). These cells commonly expressed human embryonic stem cell (hESC)/hiPSC-specific genes (POU5F1, SOX2, NANOG, LIN28, and SALL4) at a level equivalent to those of control hiPSC 201B7. The sSOM with unsupervised method demonstrated that the CSCs could be divided into three groups based on their culture conditions and original cancer tissues. Furthermore, with supervised method, sSOM nominated TMED9, RNASE1, NGFR, ST3GAL1, TNS4, BTG2, SLC16A3, CD177, CES1, GDF15, STMN2, FAM20A, NPPB, CD99, MYL7, PRSS23, AHNAK, and LOC152573 genes commonly upregulating among the CSCs compared to hiPSC, suggesting the gene signature of the CSCs.