Metabolomic analysis on blood of transgenic mice overexpressing PGC-1α in skeletal muscle
Metabolomic analysis on blood of transgenic mice overexpressing PGC-1α in skeletal muscle
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骨骼肌过表达PGC-1α转基因小鼠血液代谢组学分析
DOI：
10.1093/bbb/zbaa059
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kamei Yasutomi
中科院分区：
文献类型：
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作者：
Sugimoto Takumi;Uchitomi Ran;Hatazawa Yukino;Miura Shinji;Kamei Yasutomi
PGC-1α在运动过程中骨骼肌中表达增加，并调节许多靶基因的转录。本研究采用CE-TOFMS对骨骼肌中过表达PGC-1α的转基因小鼠（PGC-1α-Tg小鼠）的血液进行代谢组学分析。PGC-1α-Tg小鼠血液中多巴胺代谢产物高香草酸水平和骨骼肌多巴胺代谢酶基因表达均较高。5-甲氧基吲哚乙酸在PGC-1α-Tg小鼠中的血液水平也较高。PGC-1α-Tg小鼠血液中支链α-酮酸和β-丙氨酸水平较低。骨骼肌中的这些代谢物浓度较低。这些代谢物的变化可能反映了随着PGC-1α增加的骨骼肌状况，如运动。
PGC-1α expression increases in skeletal muscles during exercise and regulates the transcription of many target genes. In this study, we conducted a metabolomic analysis on the blood of transgenic mice overexpressing PGC-1α in its skeletal muscle (PGC-1α-Tg mice) using CE-TOFMS. The blood level of homovanillic acid (dopamine metabolite) and the gene expression of dopamine metabolic enzyme in the skeletal muscle of PGC-1α-Tg mice were high. The blood level of 5-methoxyindoleacetic acid was also high in PGC-1α-Tg mice. The blood levels of branched-chain α-keto acids and β-alanine were low in PGC-1α-Tg mice. These metabolites in the skeletal muscle were present in low concentration. The changes in these metabolites may reflect the skeletal muscle condition with increasing PGC-1α, such as exercise.