Over-expression of different aldehyde dehydrogenase genes in Arabidopsis thaliana confers tolerance to abiotic stress and protects plants against lipid peroxidation and oxidative stress
Over-expression of different aldehyde dehydrogenase genes in Arabidopsis thaliana confers tolerance to abiotic stress and protects plants against lipid peroxidation and oxidative stress
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DOI：
10.1111/j.1365-3040.2005.01458.x
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发表时间：
2006-06-01
影响因子：
7.3
通讯作者：
Bartels, Dorothea
中科院分区：
文献类型：
--
作者：
Kotchoni, Simeon O.;Kuhns, Christine;Bartels, Dorothea
醛脱氢酶（ALDHs）在植物受到非生物胁迫时产生的醛解毒过程中起着重要作用。在之前的研究中，我们已经证明拟南芥ALDH3I1基因在非生物胁迫下被转录激活，并且ALDH3I1基因的过表达使转基因植物具有抗逆性。拟南芥基因组包含14个ALDH基因，在不同的亚细胞区室中表达，可能参与不同的反应。本研究的目的是比较细胞质和叶绿体胁迫诱导的ALDH在不同条件下的抗逆性。我们证明了叶绿体ALDH3I1和细胞质ALDH7B4的组成性或胁迫诱导表达赋予了对渗透和氧化应激的耐受性。转基因植物的抗逆性伴随着细胞脂质过氧化产生的H2O2和丙二醛（MDA）的减少。ALDH3I1和ALDH7B4 T-DNA敲除（KO）突变体的分析证实了ALDHs参与胁迫耐受性。两个突变系对脱水和盐的敏感性均高于野生型（WT）植株。结果表明，ALDH3I1和ALDH7B4不仅具有醛解毒酶的功能，而且具有高效的活性氧（ROS）清除剂和脂质过氧化抑制酶的功能。在重组细菌蛋白中也显示了ALDHs干扰H2O2的潜力。
Aldehyde dehydrogenases (ALDHs) play a major role in the detoxification processes of aldehydes generated in plants when exposed to abiotic stress. In previous studies, we have shown that the Arabidopsis thaliana ALDH3I1 gene is transcriptionally activated by abiotic stress, and over-expression of the ALDH3I1 gene confers stress tolerance in transgenic plants. The A. thaliana genome contains 14 ALDH genes expressed in different sub-cellular compartments and are presumably involved in different reactions. The purpose of this study was to compare the potential of a cytoplasmic and a chloroplastic stress-inducible ALDH in conferring stress tolerance under different conditions. We demonstrated that constitutive or stress-inducible expression of both the chloroplastic ALDH3I1 and the cytoplasmic ALDH7B4 confers tolerance to osmotic and oxidative stress. Stress tolerance in transgenic plants is accompanied by a reduction of H2O2 and malondialdehyde (MDA) derived from cellular lipid peroxidation. Involvement of ALDHs in stress tolerance was corroborated by the analysis of ALDH3I1 and ALDH7B4 T-DNA knockout (KO) mutants. Both mutant lines exhibited higher sensitivity to dehydration and salt than wild-type (WT) plants. The results indicate that ALDH3I1 and ALDH7B4 not only function as aldehyde-detoxifying enzymes, but also as efficient reactive oxygen species (ROS) scavengers and lipid peroxidation-inhibiting enzymes. The potential of ALDHs to interfere with H2O2 was also shown for recombinant bacterial proteins.