Re-evaluation of foot process effacement in acute puromycin aminonucleoside nephrosis
Re-evaluation of foot process effacement in acute puromycin aminonucleoside nephrosis
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DOI：
10.1038/ki.1996.439
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发表时间：
1996-10-01
影响因子：
19.6
通讯作者：
Sakai, T
中科院分区：
文献类型：
--
作者：
Inokuchi, S;Shirato, I;Sakai, T
采用氢氧化钠浸渍和冷冻裂解扫描电镜（SEM）观察急性嘌呤霉素氨基核苷（PAN）肾病足突清除过程中形态学变化的顺序。扫描电镜（SEM）和透射电镜（TEM）的显微图像采用计算机形态计量学进行定量分析，并与肾功能进行相关性分析。在注射PAN后第2天，由于足突缩短和退化，狭缝长度适度减少。在第4天，膜结合的小泡散布于外板。在足突消失过程中，足细胞的基表面形成掌状结构域，代表交叉指间的细胞质区域。足细胞边界长度的减少与2 c小时肌酐清除率的减少平行。足细胞基底部掌状结构域的发育与突发性蛋白尿密切相关。我们得出结论，PAN肾病中足突的收缩和降解导致足突消失，导致掌状结构域的发展，这与足细胞脱离和大量蛋白尿有关。
The sequence of morphological changes during foot process effacement in acute puromycin aminonucleoside (PAN) nephrosis was examined by means of NaOH maceration and freeze cracking for scanning electron microscopy (SEM). The micrographs of SEM and those of transmission electron microscopy (TEM) were quantitatively analyzed by computerized morphometry, and were correlated with renal function. On day 2 after PAN injection, the slit length was moderately decreased by both shortening and degradation of the foot processes. On day 4, membrane-bounded vesicles were scattered in the lamina rara externa. During foot process effacement, the basal surface of podocytes developed palm-like domains that represented the cytoplasmic areas between interdigitation. The decrease in the length of podocyte cell borders paralleled the decrease of 2 C-hour creatinine clearance. The developement of the palm-like domains on the basal aspects of podocytes estimated by distance class analysis closely correlated with the sudden onset of proteinuria. We conclude that foot process effacement in PAN nephrosis caused by the retraction and degradation of foot processes leads to the development of palm-like domains, which is correlated with podocyte detachment as well as massive proteinuria.