Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress fromInfected Cells
Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress fromInfected Cells
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DOI：
10.1155/2011/343408
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发表时间：
2011-01-01
影响因子：
2.2
通讯作者：
Wertz, Gail W.
中科院分区：
文献类型：
--
作者：
Batonick, Melissa;Wertz, Gail W.
人呼吸道合胞病毒（HRSV）是一种有包膜的RNA病毒，从感染细胞的质膜上组装和出芽。核糖核蛋白复合物（RNP）必须与病毒基质蛋白和糖蛋白结合，以在出芽前形成新的感染性颗粒。参与HRSV组装和排出的病毒蛋白质大多未被探索。我们研究了HRSV的糖蛋白是否参与了病毒复制的后期阶段，通过利用重组病毒，其中每个单独的糖蛋白基因被删除，并替换为报告基因，以保持野生型的基因表达水平。这些工程病毒使我们能够研究糖蛋白在感染性病毒的组装和出芽中的作用。显微镜数据显示，F糖蛋白参与了糖蛋白与其他病毒蛋白在质膜上的定位。生化分析表明，F和G蛋白的缺失影响其他病毒蛋白到出芽病毒粒子的掺入。然而，有效的病毒释放不受任何糖蛋白单独或共同缺失的影响。这些研究将F和G蛋白在病毒蛋白定位和组装中的新作用归因于F和G蛋白。
Human respiratory syncytial virus (HRSV) is an enveloped RNA virus that assembles and buds from the plasma membrane of infected cells. The ribonucleoprotein complex (RNP) must associate with the viral matrix protein and glycoproteins to form newly infectious particles prior to budding. The viral proteins involved in HRSV assembly and egress are mostly unexplored. We investigated whether the glycoproteins of HRSV were involved in the late stages of viral replication by utilizing recombinant viruses where each individual glycoprotein gene was deleted and replaced with a reporter gene to maintain wild-type levels of gene expression. These engineered viruses allowed us to study the roles of the glycoproteins in assembly and budding in the context of infectious virus. Microscopy data showed that the F glycoprotein was involved in the localization of the glycoproteins with the other viral proteins at the plasma membrane. Biochemical analyses showed that deletion of the F and G proteins affected incorporation of the other viral proteins into budded virions. However, efficient viral release was unaffected by the deletion of any of the glycoproteins individually or in concert. These studies attribute a novel role to the F and G proteins in viral protein localization and assembly.