Peptide-based electrochemical approach for apoptosis evaluation.
Peptide-based electrochemical approach for apoptosis evaluation.
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DOI：
10.1016/j.bios.2014.06.035
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发表时间：
2014-12
影响因子：
12.6
通讯作者：
Peng Miao;Jian Yin;Limin Ning;Xiaoxi Li
中科院分区：
文献类型：
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作者：
Peng Miao;Jian Yin;Limin Ning;Xiaoxi Li
本文报道了一种使用肽作为识别元件在固体表面上组装凋亡细胞的策略。并首次报道了一种直接评价细胞凋亡的肽基电化学生物传感器。修饰在电极上的肽被设计成包含可以识别凋亡细胞上的外部化磷脂酰丝氨酸的序列，然后可以将细胞捕获到电极表面上。在电化学体系中，固定化细胞不仅能为电子传递提供显著的空间位阻，而且能屏蔽肽的正电荷，从而吸引带负电荷的电化学探针。因此，在凋亡细胞孵育后，获得的电化学信号显著下降，这可以用于揭示凋亡水平。该方法的实验结果与其他标准方法一致。该电化学方法具有操作简单、成本低廉、方便、灵敏等优点，在细胞凋亡评价、疗效评价和细胞生物学研究等方面具有广阔的应用前景。
This paper reports a strategy to assemble apoptotic cells on a solid surface using a peptide as the recognition element. And a peptide-based electrochemical biosensor to directly evaluate apoptosis is described for the first time. The peptide modified on an electrode is designed to contain the sequence that can recognize externalized phosphatidylserine on apoptotic cells, and can then capture the cells onto the electrode surface. In the electrochemical system, the immobilized cells can not only provide significant steric hindrance for electron transfer, but also shield the positive charges of the peptide that can attract negatively charged electrochemical probes. Therefore, the obtained electrochemical signals drop significantly after the incubation of apoptotic cells, which can be used to reveal the apoptosis level. The experimental results of this approach are well in line with other standard methods. Moreover, this electrochemical method is simple, cost-effective, convenient, sensitive, and holds great potential toward apoptosis evaluation, therapeutic effect assessment and deeper cellular biological studies.