A porous silicon-based optical interferometric biosensor
A porous silicon-based optical interferometric biosensor
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DOI：
10.1126/science.278.5339.840
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发表时间：
1997-10-31
期刊：
影响因子：
56.9
通讯作者：
Ghadiri, MR
中科院分区：
文献类型：
--
作者：
Lin, VSY;Motesharei, K;Ghadiri, MR
基于在适当衍生的多孔硅半导体薄膜的可见光反射光谱中，法布里 - 珀罗条纹的诱导波长位移，已经开发出一种生物传感器。分子的结合会引起多孔硅折射率的变化。该系统在皮摩尔和飞摩尔分析物浓度下，对小分子有机物（生物素和地高辛配基）、16 - 核苷酸DNA寡聚物以及蛋白质（链霉亲和素和抗体）的有效性和灵敏度得到了验证。该传感器对于检测单层和多层分子组装体也非常有效。
A biosensor has been developed based on induced wavelength shifts in the Fabry-Perot fringes in the visible-light reflection spectrum of appropriately derivatized thin films of porous silicon semiconductors. Binding of molecules induced changes in the refractive index of the porous silicon. The validity and sensitivity of the system are demonstrated for small organic molecules (biotin and digoxigenin), 16-nucleotide DNA oligomers, and proteins (streptavidin and antibodies) at pico- and femtomolar analyte concentrations. The sensor is also highly effective for detecting single and multilayered molecular assemblies.