THE RNA PROCESSING ENZYME RNASE MRP IS IDENTICAL TO THE TH RNP AND RELATED TO RNASE-P
THE RNA PROCESSING ENZYME RNASE MRP IS IDENTICAL TO THE TH RNP AND RELATED TO RNASE-P
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DOI：
10.1126/science.2476849
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发表时间：
1989-09-22
期刊：
影响因子：
56.9
通讯作者：
CRAFT, J
中科院分区：
文献类型：
--
作者：
GOLD, HA;TOPPER, JN;CRAFT, J
来自自身免疫性疾病患者的血清通常含有结合核糖核蛋白（RNP）的抗体。发现来自30名这样的患者的血清免疫沉淀核糖核酸酶P（RNase P），核糖核酸酶P是处理真核细胞的核和线粒体中的转移RNA转录物的5“末端所需的RNP酶。所有30个血清也免疫沉淀的核仁Th RNP，表明这两个RNP的结构相关。Th RNP的核苷酸序列分析显示，它与线粒体RNA加工酶（称为RNase MRP）的RNA组分相同。免疫沉淀Th RNP的抗体选择性地耗尽RNase MRP活性的鼠和人细胞提取物，表明Th和RNase MRP RNP是相同的。由于RNase P和RNase MRP在生化纯化过程中彼此不相关，我们认为这两种RNA加工酶共享一个共同的自身抗原多肽。
Sera from patients with autoimmune diseases often contain antibodies that bind ribonucleoproteins (RNPs). Sera from 30 such patients were found to immunoprecipitate ribonuclease P (RNase P), an RNP enzyme required to process the 5'' termini of transfer RNA transcripts in nuclei and mitochondria of eukaryotic cells. All 30 sera also immunoprecipitated the nucleolar Th RNP, indicating that the two RNPs are structurally related. Nucleotide sequence analysis of the Th RNP revealed it was identical to the RNA component of the mitochondrial RNA processing enzyme known as RNase MRP. Antibodies that immunoprecipitated the Th RNP selectively depleted murine and human cell extracts of RNase MRP activity, indicating that the Th and RNase MRP RNPs are identical. Since RNase P and RNase MRP are not associated with each other during biochemical purification, we suggest that these two RNA processing enzymes share a common autoantigenic polypeptide.