The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation.
The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation.
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DOI：
10.1073/pnas.2202669119
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发表时间：
2022-07-05
影响因子：
11.1
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中科院分区：
文献类型：
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钙（Ca 2+）是真核细胞中调节多种细胞和发育事件的通用信号，并且在植物中Ca 2+信号和生长素作用之间的联系一直被认为是缺失的。在这里，我们确定了拟南芥钙信号模块钙调素IQ基序含有蛋白质（CaM-IQM）作为一个重要的调节生长素诱导的愈伤组织和侧根形成。我们进一步证明了CaM-IQM复合物以Ca 2+依赖的方式与生长素信号抑制剂吲哚-3-乙酸诱导（IAAs）相互作用，使IAA-AXIN RESPONSE FACTOR 7相互作用不稳定，从而改变生长素的反应性。这些发现揭示了在植物再生和发育中Ca 2+信号和生长素作用之间的分子相互作用层。从离体器官诱导多能细胞团（称为愈伤组织）是体外植物再生的第一步，在此期间，植物激素生长素诱导的根发育程序的异位激活已被证明是随后的芽和根从头再生所需的。然而，其他信号是否参与控制愈伤组织的形成，从而植物再生能力，在很大程度上仍然不清楚。在这里，我们报告的拟南芥钙（Ca ~（2+））信号模块钙调素IQ基序含有蛋白质（CaM-IQM）与生长素信号相互作用，以调节愈伤组织和侧根的形成。我们表明，中断IQMs或钙调素阻滞生长素诱导的愈伤组织和侧根形成抑制生长素的反应，和钙调素-IQM复合物物理相互作用与生长素信号抑制吲哚-3-乙酸诱导（IAA）蛋白在钙离子依赖的方式。我们进一步提供证据表明，CaM 6与IAA 19的物理相互作用使IAA 19与生长素反应因子7（ARF 7）的抑制性相互作用不稳定，从而调节生长素诱导的愈伤组织形成。这些发现不仅定义了CaM-IQM介导的Ca 2+信号在愈伤组织和侧根形成中的关键作用，而且还提供了深入了解植物再生和发育过程中Ca 2+信号和生长素作用的相互作用。
Calcium (Ca2+) is a universal signal in eukaryotic cells that regulates multiple cellular and developmental events, and the link between Ca2+ signaling and auxin actions in plants has been considered to be missing. Here, we identified the Arabidopsis Ca2+ signaling module CALMODULIN IQ-MOTIF CONTAINING PROTEIN (CaM–IQM) as an important regulator of auxin-induced callus and lateral root formation. We further demonstrated that CaM–IQM complexes physically interact with auxin signaling repressors, INDOLE-3-ACETIC ACID INDUCIBLE (IAAs), in a Ca2+-dependent manner to destabilize IAA–AUXIN RESPONSE FACTOR 7 interactions and thus modify auxin responsiveness. These findings reveal a layer of molecular interplay between Ca2+ signaling and auxin actions in plant regeneration and development. Induction of a pluripotent cell mass, called callus, from detached organs is an initial step in in vitro plant regeneration, during which phytohormone auxin-induced ectopic activation of a root developmental program has been shown to be required for subsequent de novo regeneration of shoots and roots. However, whether other signals are involved in governing callus formation, and thus plant regeneration capability, remains largely unclear. Here, we report that the Arabidopsis calcium (Ca2+) signaling module CALMODULIN IQ-MOTIF CONTAINING PROTEIN (CaM–IQM) interacts with auxin signaling to regulate callus and lateral root formation. We show that disruption of IQMs or CaMs retards auxin-induced callus and lateral root formation by dampening auxin responsiveness, and that CaM–IQM complexes physically interact with the auxin signaling repressors INDOLE-3-ACETIC ACID INDUCIBLE (IAA) proteins in a Ca2+-dependent manner. We further provide evidence that the physical interaction of CaM6 with IAA19 destabilizes the repressive interaction of IAA19 with AUXIN RESPONSE FACTOR 7 (ARF7), and thus regulates auxin-induced callus formation. These findings not only define a critical role of CaM–IQM-mediated Ca2+ signaling in callus and lateral root formation, but also provide insight into the interplay of Ca2+ signaling and auxin actions during plant regeneration and development.
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影响因子：
4.3
作者：
Che, Ping;Lall, Sonia;Howell, Stephen H.
通讯作者：
Howell, Stephen H.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.2
作者：
Atta, Ramzy;Laurens, Lieve;Chriqui, Dominique
通讯作者：
Chriqui, Dominique
影响因子：
7.4
作者：
Li, Hanbing;Tiwari, Shiv B.;Guilfoyle, Tom J.
通讯作者：
Guilfoyle, Tom J.
DOI：
10.1073/pnas.1400074111
发表时间：
2014-04-08
影响因子：
11.1
作者：
Korasick, David A.;Westfall, Corey S.;Strader, Lucia C.
通讯作者：
Strader, Lucia C.