A ratiometric fluorescence sensor with broad dynamic range based on two pH-sensitive fluorophores
A ratiometric fluorescence sensor with broad dynamic range based on two pH-sensitive fluorophores
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作者：
Niu, CG;Gui, XQ;Yuan, XZ
本文介绍了一种用于 pH 测量的新型比率荧光传感器。将两种pH敏感荧光团N-烯丙基-4-(4'-甲基-哌嗪基)-1,8-萘二甲酰亚胺(AMPN)和内消旋-5,10,15,20-四-(4-烯丙氧基苯基)卟啉(TAPP)作为彼此的参考指示剂，与丙烯酰胺、甲基丙烯酸羟乙酯和二甲基丙烯酸三甘醇酯共聚在硅烷化玻璃上表面。所提出的传感器基于不同 pH 范围内两种荧光团的 pH 依赖性荧光强度。该传感器涵盖 pH 1.5-9.0 的宽动态范围。它在选择性、重现性和稳定性方面表现出令人满意的分析性能。所提出的传感器的成功制造提供了一种替代概念，可以利用两种或多种荧光团来开发覆盖广泛 pH 值的比率传感器。
This paper describes a novel ratiometric fluorescence sensor for pH measurement. Two pH-sensitive fluorophores, N-allyl-4-(4'-methyl-piperazinyl)-1,8-naphthalimide (AMPN) and meso-5,10,15,20-tetra-(4-allyloxyphenyl) porphyrin (TAPP), which served as referencing indicators for each other, were co-polymerized with acrylamide, hydroxyethyl methacrylate and triethylene glycol dimethacrylate on the silanized glass surface. The proposed sensor is based on the pH-dependent fluorescence intensities of the two fluorophores in different pH ranges. The sensor covers a broad dynamic range of pH 1.5-9.0. It exhibits satisfactory analytical performance in terms of selectivity, reproducibility and stability. The successful fabrication of the proposed sensor provides an alternative concept to utilizing two or more fluorophores for the development of ratiometric sensors covering a broad range of pH.