Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status
Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status
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DOI：
10.1007/s00425-006-0379-9
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发表时间：
2007-02
期刊：
影响因子：
4.3
通讯作者：
M. Ohnishi;T. Mimura;T. Tsujimura;N. Mitsuhashi;S. Washitani-Nemoto;M. Maeshima;E. Martinoia
中科院分区：
文献类型：
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作者：
M. Ohnishi;T. Mimura;T. Tsujimura;N. Mitsuhashi;S. Washitani-Nemoto;M. Maeshima;E. Martinoia
测定了从长春花悬浮培养细胞中分离的完整液泡对无机磷(PI)的吸收。在低PI状态下，与高PI状态相比，PI摄取被强烈地激活到液泡中。由于跨液泡膜的PI吸收与H+泵相关，我们研究了H+泵与植物PI状态的相关性。在低PI状态下，H+泵、V型H+-ATPase和H+-PPase的活性均增强。尽管H+泵增加，Western印迹分析显示质子泵蛋白的数量并没有明显增加。讨论了在低PI状态下激活PI吸收进入液泡的可能机制。
Inorganic phosphate (Pi) uptake across the vacuolar membrane of intact vacuoles isolated fromCatharanthus roseussuspension-cultured cells was measured. Under low Pi status, Pi uptake into the vacuole was strongly activated compared to high Pi status. Since Pi uptake across the vacuolar membrane is correlated with H+pumping, we examined the dependency of H+pumping on plant Pi status. Both H+pumping and the activities of the vacuolar H+-pumps, the V-type H+-ATPase and the H+-PPase were enhanced under low Pi status. Despite this increase in H+pumping, Western blot analysis showed no distinct increase in the amount of proton pump proteins. Possible mechanisms for the activation of Pi uptake into the vacuole under low Pi status are discussed.