Differentiation Kinetics of Blood Monocytes and Dendritic Cells in Macaques: Insights to Understanding Human Myeloid Cell Development.
Differentiation Kinetics of Blood Monocytes and Dendritic Cells in Macaques: Insights to Understanding Human Myeloid Cell Development.
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DOI：
10.4049/jimmunol.1500522
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发表时间：
2015-08-15
期刊：
影响因子：
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通讯作者：
Kuroda MJ
中科院分区：
文献类型：
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作者：
Sugimoto C;Hasegawa A;Saito Y;Fukuyo Y;Chiu KB;Cai Y;Breed MW;Mori K;Roy CJ;Lackner AA;Kim WK;Didier ES;Kuroda MJ
在恒河猴中使用体内BrdU脉冲追踪分析评价单核细胞和树突状细胞（DC）的发育，并通过免疫染色和流式细胞术评估血液中这些细胞的表型分析，以比较恒河猴、食蟹猴和猪尾猴以及非洲绿色猴和人之间的差异。非人灵长类物种和人类有三个单核细胞亚群，分别对应于经典、中间和非经典单核细胞的CD 14 + CD 16 −、CD 14 + CD 16+和CD 14 − CD 16+细胞。此外，目前存在两个DC亚群，BDCA-1+髓样DC和CD 123+浆细胞样DC，它们首先被证实是恒河猴血液。BrdU接种后，标记细胞首先出现在CD 14 + CD 16 −单核细胞中，然后出现在CD 14 + CD 16+细胞中，最后出现在CD 14 − CD 16+细胞中，从而定义了单核细胞成熟的不同阶段。一部分经典CD 14 + CD 16 −单核细胞逐渐表达CD 16+，成为CD 16 + CD 14+细胞，随后成熟为非经典CD 14 − CD 16+细胞亚群。BDCA-1+髓样DC和CD 123+浆细胞样DC的分化动力学与单核细胞亚群不同，表明它们的髓样细胞起源不同。使用非人灵长类动物的研究结果提供了有关单核细胞和DC的不同亚群的周转、动力学和成熟的有价值的信息，这些信息使用的方法不能在人类中容易地进行，并支持进一步的分析，以继续检查可用于解决人类疾病发病机制和干预策略的独特骨髓细胞来源。
Monocyte and dendritic cell (DC) development was evaluated using in vivo BrdU pulse-chase analyses in rhesus macaques and phenotype analyses of these cells in blood also were assessed by immunostaining and flow cytometry for comparisons between rhesus, cynomolgus, and pigtail macaques as well as African green monkeys and humans. The nonhuman primate species and humans have three subsets of monocytes, CD14+CD16−, CD14+CD16+, and CD14−CD16+ cells that correspond to classical, intermediate, and non-classical monocytes, respectively. In addition, there exist presently two subsets of DC, BDCA-1+ myeloid DC and CD123+ plasmacytoid DC that were first confirmed rhesus macaque blood. Following BrdU inoculation, labeled cells first appeared in CD14+CD16− monocytes, then in CD14+CD16+ cells, and finally in CD14−CD16+ cells, thus defining different stages of monocyte maturation. A fraction of the classical CD14+CD16− monocytes gradually expressed CD16+ to become CD16+CD14+ cells and subsequently matured into the non-classical CD14−CD16+ cell subset. The differentiation kinetics of BDCA-1+ myeloid DC and CD123+ plasmacytoid DC were distinct from the monocyte subsets, indicating differences in their myeloid cell origins. Results from studies utilizing nonhuman primates provide valuable information about the turnover, kinetics and maturation of the different subsets of monocytes and DC using approaches that cannot readily be performed in humans and support further analyses to continue examining the unique myeloid cell origins that may be applied to address disease pathogenesis mechanisms and intervention strategies in humans.