Comparison of the effects of complete and incomplete spinal cord injury on lower urinary tract function as evaluated in unanesthetized rats
Comparison of the effects of complete and incomplete spinal cord injury on lower urinary tract function as evaluated in unanesthetized rats
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DOI：
10.1016/j.expneurol.2007.07.013
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发表时间：
2007-11-01
影响因子：
5.3
通讯作者：
Wrathall, Jean R.
中科院分区：
文献类型：
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作者：
Leung, Philberta Y.;Johnson, Christopher S.;Wrathall, Jean R.
在大鼠中，相性尿道外括约肌（EUS）活动（破裂）被认为是有效排尿的关键。这在脊髓横断（txSCI）后丢失，导致功能受损。然而，麻醉可能会混淆评估EUS活动。因此，我们评估了未麻醉、受限大鼠的尿动力学参数，并比较了txSCI与临床相关的不完全性挫伤（iSCI）对下尿路功能的影响。成年雌性大鼠在T8椎体水平进行txSCI或标准化iSCI。正如预期的那样，所有受伤的大鼠最初都无法排尿，但随着时间的推移，膀胱出现了反射，iSCI大鼠比txSCI大鼠恢复得更快。损伤后2周和6周对LUT功能进行尿动力学评估。在向膀胱输注生理盐水后，对照组始终表现出膀胱的协调收缩和EUS的阶段性激活，排尿效率高（86.4 +/- 2.5%）。iSCI大鼠排尿效率降至约57%，txSCI大鼠降至约32%。然而，在两个时间点，txSCI组和iSCI组在排尿过程中都出现了两种不同的EUS活动模式：(1)与对照组相似的分相EUS活动大鼠；(2)排尿过程中仅表现出强直性EUS活动的大鼠。前者排尿效率更正常。因此，不完全性和完全性脊髓损伤后未麻醉的大鼠均可出现并检测到EUS的相性活动和与之相关的排尿效率的提高。(c) 2007爱思唯尔公司版权所有。
In rats, phasic external urethral sphincter (EUS) activity (bursting) is postulated to be crucial for efficient voiding. This has been reported to be lost after spinal cord transection (txSCI), contributing to impaired function. However, anesthesia may confound evaluating EUS activity. We therefore evaluated urodynamic parameters in unanesthetized, restrained rats and compared the effects of txSCI to that of a clinically relevant, incomplete, contusive injury (iSCI) on lower urinary tract function. Adult female rats were subjected to txSCI or standardized iSCI at the T8 vertebral level. As expected, all injured rats were initially unable to void but developed a reflex bladder with time, with iSCI rats recovering more rapidly than txSCI rats. LUT function was evaluated urodynamically at 2 and 6 weeks after injury. In response to infusion of saline into the bladder, controls consistently exhibited coordinated contraction of the bladder and activation of the EUS in a phasic pattern and had a high voiding efficiency (86.4 +/- 2.5%). Voiding efficiency of iSCI rats was reduced to approximately 57% and txSCI rats to approximately 32%. However, two different patterns of EUS activity during voiding were present in both txSCI and iSCI groups at both time points: (1) rats with phasic EUS activity, similar to controls and (2) those that only exhibited tonic EUS activity during voiding. The former had more normal voiding efficiencies. Thus, phasic EUS activity and the improved voiding efficiency associated with it can occur and can be detected in unanesthetized rats after both incomplete and complete SCI. (c) 2007 Elsevier Inc. All rights reserved.