p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation
p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation
复制标题
Kirsten 鼠肉瘤病毒的 p21 在病毒突变体中不耐热，对温度敏感以维持转化
作者：
T. Y. Shih;Howard A. Young;E. Scolnick
我们最近描述了由Kirsten （Ki-MSV）或Harvey （Ha-MSV）小鼠肉瘤病毒株转化的非生产细胞中的细胞内蛋白p21 （Shih等人，病毒学，出版中）。p21被磷酸化，并被Ki-MSV或Ha-MSV编码。在本报告中，我们比较了新合成的[35S]蛋氨酸标记的p21在由野生型Ki-MSV和Ki-MSV突变体（ts 371）转化的细胞中的热稳定性。Ki-MSV突变体在维持转化表型的一些特性所需的病毒功能中对温度敏感。在体外加热条件下，ts371 Ki-MSV编码的p21的免疫沉淀性明显优于野生型Ki-MSV的p21。目前的发现表明p21是维持Ki-MSV诱导的转化所必需的。
We have recently described an intracellular protein, p21, in nonproducer cells transformed by either the Kirsten (Ki-MSV) or Harvey (Ha-MSV) strain of murine sarcoma virus (Shih et al., Virology, in press). The p21 is phosphorylated and has been shown to be coded for by either Ki-MSV or Ha-MSV. In this report, we compare the thermal stability of the newly synthesized [35S]methionine-labeled p21 in cells transformed by the wild-type Ki-MSV or by a mutant of Ki-MSV (ts 371) which is temperature sensitive in a viral function required for the maintenance of several properties of the transformed phenotype. The immunoprecipitability of the p21 coded for by the ts 371 Ki-MSV was markedly more thermolabile than the p21 of the wild-type Ki-MSV when the cell extracts are heated in vitro. The present finding suggests that the p21 is required for the maintenance of transformation induced by Ki-MSV.