Retinal pigment epithelial cell adhesion on novel micropatterned surfaces fabricated from synthetic biodegradable polymers.
Retinal pigment epithelial cell adhesion on novel micropatterned surfaces fabricated from synthetic biodegradable polymers.
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DOI：
10.1016/s0142-9612(00)00179-4
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发表时间：
2001-02
期刊：
影响因子：
14
通讯作者：
Lichun Lu;K. Nyalakonda;Lance C. Kam;R. Bizios;A. Göpferich;A. Mikos
中科院分区：
文献类型：
--
作者：
Lichun Lu;K. Nyalakonda;Lance C. Kam;R. Bizios;A. Göpferich;A. Mikos
以聚乳酸-羟基乙酸共聚物（PLGA）和聚乙二醇-聚乳酸二嵌段共聚物（PEG/PLA）为原料，合成了具有特定化学微图案的新型生物降解聚合物基质。PLGA和PEG/PLA薄膜分别支持和抑制视网膜色素上皮（RPE）细胞增殖，7天后相应的细胞密度为352900和850 cells/cm 2（从初始接种密度15000 cells/cm 2）。微接触印刷技术用于定义由PEG/PLA区域（宽度为50 μ m）包围和分隔的圆形（直径为50 μ m）PLGA结构域阵列。还制备了由PLGA区域包围的PEG/PLA环形结构域组成的反向图案。这两个微图案化的表面被证明会影响初始RPE细胞附着，限制细胞扩散，并在8小时的实验期间促进特征性立方细胞形态。相比之下，在普通PLGA（对照膜）上的RPE细胞被拉长并且呈现成纤维细胞样。相反的模式具有连续的PLGA区域，其允许细胞-细胞相互作用，从而更高的细胞粘附。这些结果证明了制造微图案化的合成生物可降解聚合物表面以控制RPE细胞形态的可行性。
Novel synthetic biodegradable polymer substrates with specific chemical micropatterns were fabricated from poly(dl-lactic-co-glycolic acid) (PLGA) and diblock copolymers of poly(ethylene glycol) and poly(dl-lactic acid) (PEG/PLA). Thin films of PLGA and PEG/PLA supported and inhibited, respectively, retinal pigment epithelial (RPE) cell proliferation, with a corresponding cell density of 352900 and 850cells/cm2after 7 days (from an initial seeding density of 15000cells/cm2). A microcontact printing technique was used to define arrays of circular (diameter of 50μm) PLGA domains surrounded and separated by regions (width of 50μm) of PEG/PLA. Reversed patterns composed of PEG/PLA circular domains surrounded by PLGA regions were also fabricated. Both micropatterned surfaces were shown to affect initial RPE cell attachment, limit cell spreading, and promote the characteristic cuboidal cell morphology during the 8-h period of the experiments. In contrast, RPE cells on plain PLGA (control films) were elongated and appeared fibroblast-like. The reversed patterns had continuous PLGA regions that allowed cell–cell interactions and thus higher cell adhesion. These results demonstrate the feasibility of fabricating micropatterned synthetic biodegradable polymer surfaces to control RPE cell morphology.