Isolation of marine meiofauna from sandy sediments: from decanting to DNA extraction.
Isolation of marine meiofauna from sandy sediments: from decanting to DNA extraction.
复制标题
从沙质沉积物中分离海洋小型动物：从倾析到 DNA 提取。
DOI：
10.1038/nprot.2010.157
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Fonseca V
中科院分区：
文献类型：
--
作者：
Fonseca V
This protocol describes the separation of marine meiofauna from sediment and subsequent environmental DNA extraction. In this study meiofauna samples were taken with a 45 mm core from the upper 5 to 10 cm of sediment layer. Separation from sediment was achieved using a decantation process followed by isolation from fine silt using repetitive centrifugation steps with a 1.16 specific gravity (sg) LUDOX-TM solution. Meiofauna were deliberately separated from macrofauna by using a 1 mm sieve on top of a bottle-top sterile 45 µM sieve. High quality DNA was subsequently obtained using the QIAamp DNA Blood Maxi Kit (Qiagen) with minor adjustments to the manufacturer’s protocol. This procedure allowed efficient isolation of meiofaunal representatives from marine sediments and also extraction of high quality environmental DNA that can be used for downstream metagenetic analysis.
