TRANSCRIPTIONAL ACTIVATION OF THE TRANSLOCATED C-MYC ONCOGENE IN BURKITT-LYMPHOMA
TRANSCRIPTIONAL ACTIVATION OF THE TRANSLOCATED C-MYC ONCOGENE IN BURKITT-LYMPHOMA
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DOI：
10.1073/pnas.80.3.820
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
CROCE, CM
中科院分区：
文献类型：
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作者：
ERIKSON, J;ARRUSHDI, A;CROCE, CM
在t（8;14）染色体易位的Burkitt淋巴瘤中，VH基因从14号染色体易位到8号染色体易位，c-菌原基因从8号染色体易位到14号染色体易位。c-myc基因与C.mu的关联。Ig基因在之前研究的部分伯基特淋巴瘤中被观察到，但不是全部。在P3HR-1伯基特淋巴瘤细胞系中研究了人H链位点和c-myc基因的组织。由于小鼠/P3HR-1体细胞杂交只保留14q +染色体而不保留其他人类染色体，因此含有人类C.mu。和C.gamma。在P3HR-1细胞中，14号染色体上的断点在远端的CUm和C.mu.之间。和VH。人14号染色体的断点在不同的伯基特淋巴瘤细胞系中是不同的。在P3HR-1细胞系中，易位到14号染色体的人c-myc癌基因未与c-myc癌基因重组。基因。因此，人类8号染色体上的断点在不同的伯基特淋巴瘤细胞系中也可能不同，因为c-myc基因与C.mu基因的DNA重排。基因仅在其他地方研究的一些伯基特淋巴瘤细胞系中观察到。在易位c-myc癌基因重排和未重排的Burkitt淋巴瘤中观察到高水平的c-myc癌基因转录本。c-myc癌基因易位到人类14号染色体上的重链位点显然足以激活其转录，并且可能是导致肿瘤发生途径的重要步骤。
Translocations of VH genes from chromosome 14 to chromosome 8 and of the c-myconcogene from chromosome 8 to chromosome 14 occur in Burkitt lymphomas with the t(8;14) chromosome translocation. An association of the c-myc gene with the C.mu. Ig gene was observed in some but not all Burkitt lymphomas studied previously. The organization of the human H chain locus and of the c-myc gene was investigated in the P3HR-1 Burkitt lymphoma cell line. Because mouse/P3HR-1 somatic cell hybrids that retain only the 14q + chromosome and no other human chromosome contain the human C.mu. and C.gamma. genes but not VH genes, the breakpoint on chromosome 14 in P3HR-1 cells is distal to CUm and between C.mu. and VH. The breakpoint of human chromosome 14 differs in different Burkitt lymphoma cell lines. The human c-myc oncogene translocated to chromosome 14 in the P3HR-1 cell line is not recombined with the C.mu. gene. The breakpoint on human chromosome 8 may therefore also differ in different Burkitt lymphoma cell lines, because DNA rearrangement of the c-myc gene with the C.mu. gene was observed in only some of the Burkitt lymphoma cell lines studied elsewhere. High levels of transcripts of the c-myc oncogene were observed in Burkitt lymphomas with translocated c-myc oncogenes both rearranged and unrearranged. The translocation of a c-myc oncogene to the heavy chain locus on human chromosome 14 is apparently sufficient for its transcriptional activation and may be an essential step in the pathway leading to neoplasia.