Infection prevalence of Sodalis symbionts among stinkbugs.
Infection prevalence of Sodalis symbionts among stinkbugs.
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DOI：
10.1186/s40851-014-0009-5
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发表时间：
2015
影响因子：
2.7
通讯作者：
Fukatsu T
中科院分区：
文献类型：
--
作者：
Hosokawa T;Kaiwa N;Matsuura Y;Kikuchi Y;Fukatsu T
不同的昆虫和其他生物体与微生物共生体相关联，微生物共生体通常显著地促进其宿主的生长和存活和/或以各种方式显著地影响其宿主的表型。Sodalis glossinidius首先被鉴定为采采蝇的兼性细菌共生体，最近的研究表明，Sodalis-同源细菌包括不同的生态位，从自由生活的细菌到兼性共生体，再到与多种昆虫相关的专性共生体。尽管潜在的生态和进化相关性的Sodalis共生体，其感染率在自然昆虫种群一直调查甚少。调查了17科77属108种310个居群960头不同种类的蝽类及其近缘陆生异翅蝽类昆虫与扁蝽共生体的感染情况。诊断性PCR检测到相对较低的Sodalis共生体的感染频率：13.6%（14/103）的物种，7.5%（22/295）的种群，和4.3%（35/822）的个体的蝽类（Urostylididae的家庭除外）。在尾柱蝽中，显著的是，Sodalis共生体表现出非常高的感染频率：100%（5/5）的物种，100%（15/15）的种群，和94.2%（130/138）的个人，我们检查。基于细菌16 S rRNA基因序列的分子系统发育分析表明，所有共生体都属于Sodalis-同源细菌的分支，而共生体的系统发育并不能反映其寄主的系统发育。值得注意的是，Sodalis共生体的urostylidid的蝽没有集群与Sodalis共生体的其他蝽类的发育，这表明他们不同的进化轨迹。相对较低的感染频率和整体主机共生系统发育的不一致性表明，Sodalis共生体，在一般情况下，在大多数的臭虫组的兼性共生协会。另一方面，可以想象，尽管是推测性的，Sodalis共生体可能对它们的宿主Urostylidididae的臭虫发挥一些实质性的生物学作用。本文的在线版本（doi：10.1186/s40851-014-0009-5）包含补充材料，可供授权用户使用。
Diverse insects and other organisms are associated with microbial symbionts, which often significantly contribute to growth and survival of their hosts and/or drastically affect phenotypes of their hosts in a variety of ways. Sodalis glossinidius was first identified as a facultative bacterial symbiont of tsetse flies, and recent studies revealed that Sodalis-allied bacteria encompass diverse ecological niches ranging from free-living bacteria through facultative symbionts to obligate symbionts associated with a diverse array of insects. Despite potential ecological and evolutionary relevance of the Sodalis symbionts, their infection prevalence in natural insect populations has been poorly investigated. Here we surveyed diverse stinkbugs and allied terrestrial heteropteran bugs, which represented 17 families, 77 genera, 108 species, 310 populations and 960 individuals, for infection with the Sodalis symbionts. Diagnostic PCR detected relatively low infection frequencies of the Sodalis symbionts: 13.6% (14/103) of the species, 7.5% (22/295) of the populations, and 4.3% (35/822) of the individuals of the stinkbugs except for those belonging to the family Urostylididae. Among the urostylidid stinkbugs, strikingly, the Sodalis symbionts exhibited very high infection frequencies: 100% (5/5) of the species, 100% (15/15) of the populations, and 94.2% (130/138) of the individuals we examined. Molecular phylogenetic analysis based on bacterial 16S rRNA gene sequences revealed that all the symbionts were placed in the clade of Sodalis-allied bacteria while the symbiont phylogeny did not reflect the systematics of their stinkbug hosts. Notably, the Sodalis symbionts of the urostylidid stinkbugs were not clustered with the Sodalis symbionts of the other stinkbug groups on the phylogeny, suggesting their distinct evolutionary trajectories. The relatively low infection frequency and the overall host-symbiont phylogenetic incongruence suggest that the Sodalis symbionts are, in general, facultative symbiotic associates in the majority of the stinkbug groups. On the other hand, it is conceivable, although speculative, that the Sodalis symbionts may play some substantial biological roles for their host stinkbugs of the Urostylididae. The online version of this article (doi:10.1186/s40851-014-0009-5) contains supplementary material, which is available to authorized users.